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黑籽重楼的生药学研究
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| 篇名： | 黑籽重楼的生药学研究 |
| TITLE： | |
| 摘要： | 目的：对重楼药材原植物黑籽重楼进行系统的生药学研究。方法：对黑籽重楼的植物形态、药材特征、药材横切面及粉末进行观察；以重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ为对照品对药材进行薄层鉴别，采用高效液相色谱法测定黑籽重楼中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ的含量。结果：黑籽重楼的原植物特征包括具有侧膜胎座，果开裂，无外种皮，于一侧包以深红色多汁的鸡冠状假种皮；查阅文献，黑籽重楼与云南重楼、七叶一枝花在药材性状上的区别主要表现在表面颜色、形状、环纹、断面上，气味上没有明显的差异；黑籽重楼的横切面结构、粉末特征与云南重楼、七叶一枝花没有明显差异；经薄层色谱鉴别，在黑籽重楼根茎中都含有重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ，含量分别为0.117％、0.081％、0.114％、0.204％；重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ的总量为0.516％，与药典标准（含重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ的总量不得少于0.60％）存在差异。结论：本研究可为黑籽重楼的鉴定及进一步研究提供科学依据。 |
| ABSTRACT： | OBJECTIVE： Conduct a systematic pharmacognostical study on herbs of Paris thibetica Franch. METHODS： The plant morphology of Paris thibetica Franch， characteristics， cross section and powder were all observed. The polyphyllin Ⅰ， Ⅱ， Ⅵ and Ⅶ were used as reference substance for TLC. HPLC method was used to determine the contents of polyphyllin Ⅰ， Ⅱ， Ⅵ and Ⅶ in Paris thibetica Franch. RESULTS： The original plant characteristic of Paris thibetica Franch was the lateral membrane placenta， the fruits were cracked without outer seed coat， a dark red juicy cockatoo aril was wrapped on one side. After literature review， the character differences of Paris polyphylla Smith var. yunnanensis （Franch.） Hand.-Mazz. and Paris polyphylla Smith var. chinensis （Franch.） Hara were mainly manifested in the surface color， shape， ring pattern and cross section， but there was no obvious difference in odor. There was no significant difference in the transverse section structure and powder characteristics of P. polyphylla Smith var. Yunnanensis （Franch.） Hand. Mazz and P. polyphylla Smith var. chinensis （Franch.） Hara. TLC identification showed that polyphyllin Ⅰ， Ⅱ， Ⅵ and Ⅶ were all found in Paris thibetica Franch， the contents were 0.117％， 0.081％， 0.114％， 0.204％， respectively. Total content of polyphyllin Ⅰ， Ⅱ， Ⅵ and Ⅶ was 0.516％， which was different from the standard value of China Pharmacopoeia （total content of them was no less than 0.60％）. CONCLUSIONS： This study can provide scientific basis for the identification and further research of Paris thibetica Franch. |
| 期刊： | 2018年第29卷第23期 |
| 作者： | 赵兴蕊，刘常逊，杨发建，王洪云 |
| AUTHORS： | ZHAO Xingrui，LIU Changxun，YANG Fajian，WANG Hongyun |
| 关键字： | 黑籽重楼；性状鉴别；显微鉴别；理化鉴别；高效液相色谱法 |
| KEYWORDS： | Paris thibetica Franch； Characteristics identification； Microscopic identification； Physicochemical identification； HPLC |
| 阅读数： | 509 次 |
| 本月下载数： | 6 次 |
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