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姜黄素对高糖培养下大鼠神经胶质瘤细胞C6中p53、Siah-1和突触素蛋白表达的影响
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| 篇名： | 姜黄素对高糖培养下大鼠神经胶质瘤细胞C6中p53、Siah-1和突触素蛋白表达的影响 |
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| 摘要： | 目的：体外研究姜黄素对高糖培养下大鼠神经胶质瘤细胞C6中抑癌基因p53、E3泛素连接酶Siah-1和认知相关蛋白突触素（Synaptophysin）表达的影响。方法：采用CCK-8法检测10、20、30、40、50、60、70、80、90、100 μmol/L姜黄素在高糖培养下作用C6细胞24、48 h后的细胞存活率。采用Western blot法和实时荧光定量聚合酶链式反应（RT- PCR）法检测作用24 h后正常对照组（无任何处理）、高糖培养组和姜黄素低、高浓度组（高糖+10、30 μmol/L姜黄素）细胞中p53、Siah-1、Synaptophysin蛋白及mRNA的表达情况；另同法检测高糖培养下小干扰RNA（siRNA）沉默C6细胞中p53基因后对Siah-1蛋白及mRNA表达的影响。结果：姜黄素在10～40 μmol/L浓度范围内对C6细胞存活率无明显影响，大于40 μmol/L浓度后呈浓度依赖性降低细胞存活率。与正常对照组比较，高糖培养组C6细胞中p53、Siah-1蛋白及mRNA表达增强，Synaptophysin蛋白及mRNA表达减弱，差异均有统计学意义（P＜0.05）；与高糖培养组比较，姜黄素低、高浓度组C6细胞中p53、Siah-1蛋白及mRNA表达减弱，Synaptophysin蛋白及mRNA表达增强，差异均有统计学意义（P＜0.05）。高糖培养下，沉默p53基因后的C6细胞中Siah-1蛋白及mRNA表达均明显减弱（P＜0.05）。结论：高糖条件下，姜黄素可通过下调C6细胞中p53、Siah-1表达，进而上调Synaptophysin表达。 |
| ABSTRACT： | OBJECTIVE： To study the effects of curcumin on the expression of tumor suppressor gene p53， E3 ligase Siah-1 and cognitive associated protein Synaptophysin in glioma cells C6 of rats cultured in high glucose. METHODS： CCK-8 assay was used to detect survival rate of C6 cells after treated with 10， 20， 30， 40， 50， 60， 70， 80， 90， 100 μmol/L curcumin for 24， 48 h under high glucose environment. Western blot method and RT-PCR method were adopted to detect the protein and mRNA expressions of p53， Siah-1 and Synaptophysin in C6 cells of normal control group （without any treatment）， high glucose treatment group， curcumin low-concentration and high-concentration groups （high glucose+10， 30 μmol/L curcumin） after cultured for 24 h. Same method was used to determine the effects of P53 gene of C6 cells on protein and mRNA expression of Siah-1 after transfected with siRNA under high glucose environment. RESULTS： Curcumin had no significant effect on the survival rate of C6 cells in the range of 10-40 μmol/L. The survival rate of C6 cells decreased significantly in a concentration-dependent manner when the concentration of curcumin was higher than 40 μmol/L. Compared with normal control group， the protein and mRNA expressions of p53 and Siah-1 in C6 cells were enhanced in high glucose treatment group， while the protein and mRNA expressions of Synaptophysin were decreased， with statistical significance （P＜0.05）. Compared with high glucose treatment group， the protein and mRNA expressions of p53 and Siah-1 in C6 cells were decreased in curcumin low-concentration and high-concentration groups， while the protein and mRNA expressions of Synaptophysin were enhanced， with statistical significance （P＜0.05）. Under treated with high glucose， the protein and mRNA expressions of Siah-1 in C6 cells were decreased significantly after silencing p53 gene （P＜0.05）. CONCLUSIONS： Under high glucose environment， curcumin can up-regulate the expression of Synaptophysin in C6 cells by down-regulating the expression of p53 and Siah-1. |
| 期刊： | 2018年第29卷第23期 |
| 作者： | 刘晓惠，赵延欣，刘学源 |
| AUTHORS： | LIU Xiaohui，ZHAO Yanxin，LIU Xueyuan |
| 关键字： | 姜黄素；高糖；p53；大鼠神经胶质瘤细胞C6；突触素 |
| KEYWORDS： | Curcumin； High glucose； p53； Glioma cells C6 of rats； Synaptophysin |
| 阅读数： | 371 次 |
| 本月下载数： | 10 次 |
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