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蒙药蓝盆花乙醇提取物的血清药物化学研究
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| 篇名： | 蒙药蓝盆花乙醇提取物的血清药物化学研究 |
| TITLE： | |
| 摘要： | 目的：初步探讨蒙药蓝盆花乙醇提取物在大鼠体内的血中移行成分，为阐明该药的药效物质基础提供参考。方法：将10只大鼠随机分成给药组（以生药量计为0.3 g/100 g）和空白组（0.5％羧甲基纤维素钠溶液），每组5只，灌胃给药，给药量均为2 mL/100 g，早、晚8：00各给药1次，连续给药3 d。末次给药30 min后，肝门静脉取血，经有机溶剂沉淀法分别制得含药血清和空白血清样品。以蓝盆花原药材为对照，采用超高效液相色谱-串联质谱法进行测定，通过比较3种样品的化学成分差异，再结合化合物对照品和文献数据，鉴定蓝盆花乙醇提取物灌胃后大鼠体内的血中移行成分。结果：共发现了39个血中移行成分，其中原型成分17个、代谢成分22个，原型成分主要为黄酮类及苯丙酸类，在大鼠体内多发生甲基化、硫酸化与葡萄糖醛酸化等反应。结论：初步确定了大鼠灌胃蓝盆花乙醇提取物后的入血成分，为该药材进一步的体内代谢研究奠定了基础。 |
| ABSTRACT： | OBJECTIVE： To preliminarily investigate the constituents absorbed into the blood of ethanol extract from Mongolia medicine Scabiosa comosa in rats， and to provide reference for clarifying the effective substance basis of the medicine. METHODS： Ten rats were randomly divided into administration group （0.3 g/100 g by crude drug） and blank group （0.5％ CMC-Na solution）， with 5 rats in each group. The were given relevant medicine intragastrically 2 mL/100 g， at 8：00 morning and evening， for consecutive 3 d. 30 min after last administration， blood samples were collected from hepatic portal vein. The containing serum samples and blank serum samples were prepared by organic solvent precipitation. UPLC-MS/MS method was adopted by using S. comosa as control. Through comparing chemical components of 3 kinds of samples， constituents of ethanol extract from S. comosa in rats after intragastric administration were identified on the basis of compound control and literature data. RESULTS： Totally 39 constituents absorbed into blood were found， of which 17 were prototype components and 22 related metabolites. The prototype components were mainly flavonoids and phenylpropionic acid. Methylation， sulfation and glucose aldehyde reactions were observed in rats. CONCLUSIONS： The constituents into the blood of ethanol extract from S. comosa in rats are confirmed preliminarily after intragastric administration， which lay a foundation for further metabolism study in vivo. |
| 期刊： | 2018年第29卷第21期 |
| 作者： | 马飞祥，薛培凤#，韩盟帝，王媛媛 |
| AUTHORS： | MA Feixiang，XUE Peifeng，HAN Mengdi，WANG Yuanyuan |
| 关键字： | 蓝盆花；血清药物化学；超高效液相色谱-串联质谱法；血中移行成分；大鼠 |
| KEYWORDS： | Scabiosa comosa； Serum pharmacochemistry； UPLC-MS/MS； Constituents absorbed into the blood； Rats |
| 阅读数： | 254 次 |
| 本月下载数： | 4 次 |
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