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重组腺病毒Ad-GFP-C197对3种肿瘤细胞的抑制作用研究
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| 篇名： | 重组腺病毒Ad-GFP-C197对3种肿瘤细胞的抑制作用研究 |
| TITLE： | |
| 摘要： | 目的：研究以腺病毒载体系统装载人端粒酶逆转录酶C端片段（C197）制成的重组腺病毒Ad-GFP-C197对3种肿瘤细胞增殖的抑制作用。方法：采用HEK293细胞扩增并纯化Ad-GFP-C197。采用Ad-GFP-C197分别感染人胃癌细胞SGC7901、人乳腺癌细胞MCF7和人结直肠癌细胞CaCO2。以空白腺病毒载体（Ad-GFP）为参比，采用Western blot法检测Ad-GFP-C197感染后3种肿瘤细胞中C197的蛋白表达水平；采用MTT法检测Ad-GFP-C197感染后对3种肿瘤细胞的抑制作用，绘制细胞增殖曲线并计算增殖抑制率。结果：Ad-GFP感染的3种肿瘤细胞中均未检测到C197蛋白表达，而Ad-GFP-C197感染的上述细胞中C197蛋白均有明显表达；Ad-GFP-C197感染后3种肿瘤细胞的增殖曲线随时间延长而呈明显抑制现象，其增殖抑制率最高可达37.31％～41.42％。结论：Ad-GFP-C197对SGC7901细胞、MCF7细胞、CaCO2细胞增殖均有明显的抑制作用，且起效迅速，能够克服其他端粒酶抑制剂起效缓慢的缺点。 |
| ABSTRACT： | OBJECTIVE： To study the inhibitory effects of recombinant adenovirus Ad-GFP-C197， which prepared by adenovirus vector system-loading human telomerase reverse transcriptase （hTERT） C fragment （C197）， on the proliferation of 3 kinds of tumor cells in vitro. METHODS： Ad-GFP-C197 was amplified and purified with HEK293 cells. Human gastric cancer cells SGC7901， human breast cancer cells MCF7 and human colorectal cancer cells CaCO2 were infected by Ad-GFP-C197 respectively. Using blank adenovirus carrier （Ad-GFP） as reference， the protein expression of C197 in 3 kinds of tumor cells infected by Ad-GFP-C197 was detected by Western blot assay. The inhibitory effects of Ad-GFP-C197 on 3 kinds of tumor cells were detected by MTT assay. The cell proliferation curve was drawn and the proliferation inhibition rate was calculated. RESULTS： The protein expression of C197 was not detected in 3 kinds of tumor cells infected by Ad-GFP， while significant protein expression of C197 was found in above cells infected by Ad-GFP-C197. The proliferation curves of the 3 kinds of tumor cells infected by Ad-GFP-C197 were significantly inhibited with the time extended， and the proliferation inhibitory rate reached 37.31％-41.42％. CONCLUSIONS： Ad-GFP-C197 shows significant inhibitory effects on the proliferation of SGC7901， MCF7 and CaCO2 cells， which is rapid to make up for the slow effect of other telomerase inhibitors. |
| 期刊： | 2018年第29卷第20期 |
| 作者： | 邬贤，黄莹，李舒婕，段萍萍，米鹏程，陈文瑛 |
| AUTHORS： | WU Xian，HUANG Ying，LI Shujie，DUAN Pingping，MI Pengcheng，CHEN Wenying |
| 关键字： | 人端粒酶逆转录酶；端粒酶抑制剂；重组腺病毒；Ad-GFP-C197；肿瘤细胞；细胞增殖 |
| KEYWORDS： | hTERT； Telomerase inhibitor； Recombinant adenovirus； Ad-GFP-C197； Tumor cell； Cell proliferation |
| 阅读数： | 311 次 |
| 本月下载数： | 5 次 |
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