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溪黄草水提物对四氯化碳诱导大鼠肝纤维化的保护作用及机制研究
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| 篇名： | 溪黄草水提物对四氯化碳诱导大鼠肝纤维化的保护作用及机制研究 |
| TITLE： | |
| 摘要： | 目的：研究溪黄草水提物（RWE）对四氯化碳（CCl4）诱导大鼠肝纤维化（HF）的保护作用及其机制。方法：将60只雄性SD大鼠随机分为正常组、模型组、秋水仙碱组（0.12 mg/kg）和RWE低、中、高剂量组（4、8、16 g/kg，以生药量计），每组10只。除正常组大鼠腹腔注射橄榄油外，其余各组大鼠均腹腔注射40％ CCl4橄榄油溶液以复制HF模型。自造模第1天起，各给药组大鼠均灌胃相应药物（10 mL/kg），正常组和模型组大鼠均灌胃等体积水，每天1次，连续6周。给药结束后，采用生化法或酶联免疫吸附测定法检测大鼠血清中丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、透明质酸（HA）、层粘连蛋白（LN）、Ⅲ型前胶原（PCⅢ）、Ⅳ型胶原（Ⅳ-C）的含量以及肝组织中丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）、IL-1β的含量或活性；采用苏木精-伊红染色观察大鼠肝组织病理学变化；采用蛋白质印迹法检测大鼠肝组织中α-平滑肌肌动蛋白（α-SMA）、转化生长因子β1（TGF-β1）蛋白表达情况。结果：与正常组比较，模型组大鼠血清中ALT、AST、HA、LN、PCⅢ、Ⅳ-C的含量以及肝组织中MDA、TNF-α、IL-6、IL-1β的含量均显著升高（P＜0.01），肝组织中SOD、GSH-Px的活性均显著降低（P＜0.01）；肝组织纤维化明显；肝组织中α-SMA、TGF-β1蛋白的相对表达量均显著升高（P＜0.01）。与模型组比较，秋水仙碱组和RWE各剂量组大鼠血清中ALT、AST、HA、LN、PCⅢ、Ⅳ-C的含量以及肝组织中MDA、TNF-α、IL-6的含量，秋水仙碱组和RWE中、高剂量组大鼠肝组织中IL-1β的含量均显著降低（P＜0.05或P＜0.01），秋水仙碱组和RWE各剂量组大鼠肝组织中SOD、GSH-Px的活性均显著增强（P＜0.05或P＜0.01）；肝组织纤维化程度明显减轻；肝组织中α-SMA、TGF-β1蛋白的相对表达量均显著降低（P＜0.01）。结论：RWE对CCl4诱导的HF模型大鼠具有一定的保护作用，其作用机制可能与调节脂质代谢、减轻肝脂质过氧化损伤、抗氧化应激反应、抑制炎症因子释放及TGF-β1蛋白表达等有关。 |
| ABSTRACT： | OBJECTIVE： To study the protective effects and the mechanism of Rabdosia serra water extract （RWE） on hepatic fibrosis （HF） induced by carbon tetrachloride （CCl4） in rats. METHODS： Sixty male SD rats were randomly divided into normal group， model group， colchicine group （0.12 mg/kg）， and RWE low-dose， medium-dose and high-dose groups （4， 8， 16 g/kg， by crude drug）， with 10 rats in each group. Except for intraperitoneal injection of olive oil for normal group， other groups were given 40％ CCl4 olive oil solution intraperitoneally to induce HF model. Since the first day of modeling， each treatment group was given relevant medicine （10 mL/kg） intragastrically， while normal group and model group were given constant volume of water intragastrically， once a day， for consecutive 6 weeks. After medication， biochemical process or ELISA were used to determine the contents of ALT， AST， HA， LN， PCⅢ and Ⅳ-C in serum， the activities or contents of SOD， GSH-Px， MDA， TNF-α， IL-6 and IL-1β in liver tissue. Pathological changes of liver tissue in rats were observed by HE staining. The expression of α-SMA and TGF-β1 in liver tissue were detected by Western blot. RESULTS： Compared with normal group， the contents of ALT， AST， LN， HA， PCⅢ and Ⅳ-C in serum， the contents of MDA， TNF-α， IL-6 and IL-1β in liver tissue were all increased significantly in model group （P＜0.01）； the activities of SOD and GSH-Px in liver tissue were decreased significantly （P＜0.01）. Liver fibrosis was obvious， and the relative expression of α-SMA and TGF-β1 were increased significantly （P＜0.01）. Compared with model group， the contents of ALT， AST， HA， LN， PCⅢ and Ⅳ-C in serum as well as the contents of MDA， TNF-α and IL-6 in liver tissue in colchicines group and RWE groups， the contents of IL-1β in liver tissue of rats in colchicines group， RWE medium-dose and high-dose groups were all decreased significantly （P＜0.05 or P＜0.01）. The activities of SOD and GSH-Px in liver tissue of rats were increased significantly in colchicines group and RWE groups （P＜0.05 or P＜0.01）. The fibrosis degree of liver tissue was significantly reduced， while the relative expression of α-SMA and TGF-β1 decreased significantly （P＜0.01）. CONCLUSIONS： RWE can protect CCl4-induced HF model rats， the mechanism of which may be associated with regulating lipid metabolism， relieving liver lipid peroxidation injury and anti-oxidative stress response， inhibiting the release of inflammatory factors and the expression of TGF-β1. |
| 期刊： | 2018年第29卷第20期 |
| 作者： | 许琼梅，李跃龙，曹后康，陈春，王刚，朱依谆，张可锋 |
| AUTHORS： | XU Qiongmei，LI Yuelong，CAO Houkang，CHEN Chun，WANG Gang，ZHU Yizhun，ZHANG Kefeng |
| 关键字： | 溪黄草；水提物；肝纤维化；四氯化碳；氧化应激；炎症因子；转化生长因子β1；大鼠 |
| KEYWORDS： | Rabdosia serra； Water extract； Hepatic fibrosis； Carbon tetrachloride； Oxidative stress； Inflammatory factor； TGF-β1； Rats |
| 阅读数： | 390 次 |
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