七氟烷对小鼠空间记忆能力和海马组织BDNF、TrkB表达的影响-中国药房

七氟烷对小鼠空间记忆能力和海马组织BDNF、TrkB表达的影响

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篇名：	七氟烷对小鼠空间记忆能力和海马组织BDNF、TrkB表达的影响
TITLE：
摘要：	目的：研究七氟烷对小鼠空间记忆能力和海马组织中脑源性神经营养因子（BDNF）、酪氨酸激酶B（TrkB）表达的影响。方法：将54只C57BL/6小鼠随机分为麻醉组和对照组，每组27只。上述2组又分别分为麻醉后第1 d（第一阶段）、第7 d（第二阶段）、第28 d（第三阶段）3个亚组，每亚组9只。麻醉组小鼠置于麻醉箱中，给予七氟烷（初始体积分数为5％，维持1 min，后降至2.5％，维持120 min），完成麻醉后复苏；对照组小鼠始终给予纯氧。每阶段均进行Morris水迷宫实验，每阶段结束后分别采用聚合酶链式反应法、酶联免疫吸附法测定海马组织中BDNF、TrkB mRNA和蛋白表达水平。结果：与对照组比较，第一阶段麻醉组小鼠平台象限停留时间显著延长，平台象限停留时间百分比显著升高，差异均有统计学意义（P＜0.05），穿越平台次数差异无统计学意义（P＞0.05）；第二、三阶段麻醉组小鼠平台象限停留时间、平台象限停留时间百分比、穿越平台次数差异均无统计学意义（P＞0.05）。3个阶段中，麻醉组小鼠海马组织中BDNF、TrkB mRNA和蛋白表达水平差异均无统计学意义（P＞0.05）。结论：七氟烷麻醉可增强麻醉后早期小鼠空间记忆能力，且不影响其海马组织中BDNF和TrkB的合成。
ABSTRACT：	OBJECTIVE： To study the effects of sevoflurane on spatial memory ability and expression of brain-derived neurotrophic factor （BDNF） and tyrosine kinase B （TrkB） in hippocampus of mice. METHODS： A total of 54 C57BL/6 mice were randomly divided into anesthesia group and control group， with 27 rats in each group. Above 2 groups were divided into first day （first stage）， seventh day （second stage）， twenty-eighth day （third stage） sub-groups after anaesthesia， with 9 mice in each. In anesthesia group， mice were placed in a narcotic box， given sevoflurane 5％ for 1 min and then decreased to 2.5％ for 120 min； mice recoveried after anaesthesia completion. In control group， mice were always given pure oxygen. Morris water maze test was conducted in each stage. mRNA and protein expression levels of BDNF and TrkB in mice hippocampus were determined by PCR and ELISA after each stage. RESULTS： Compared with control group， platform quadrant and retention time of mice was prolonged significantly in first stage of anesthesia group， and the percentage of platform quadrant time was increased significantly， with statistical significance （P＜0.05）， but there was no significant change in the times of crossing platform （P＞0.05）. In second and third stage， there was no significant change in platform quadrant and retention time， the percentage of platform quadrant time or the times of crossing platform in anesthesia group （P＞0.05）. There was no significant change in mRNA and protein expression levels of BNDF and TrkB in mice hippocampus of anesthesia group （P＞0.05）. CONCLUSIONS： Sevoflurane anesthesia can strengthen spatial memory ability of mice in the early stage after anesthesia， but does not influence the generation of BDNF and TrkB in hippocampus.
期刊：	2018年第29卷第14期
作者：	许坤，辛怡纯，陈恳，陆志俊
AUTHORS：	XU Kun，XIN Yichun，CHEN Ken，LU Zhijun
关键字：	七氟烷；小鼠；空间记忆；Morris水迷宫；脑源性神经营养因子；酪氨酸激酶B
KEYWORDS：	Sevoflurane； Mice； Spatial memory； Morris water maze； Brain-derived neurotrophic factor； Tyrosine kinase B
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