返回 
加入收藏
复方鹿角霜的急性毒性研究
x
请在关注微信后，向客服人员索取文件
| 篇名： | 复方鹿角霜的急性毒性研究 |
| TITLE： | |
| 摘要： | 目的：研究复方鹿角霜的急性毒性。方法：将40只SPF级昆明小鼠分为空白对照组（等体积羧甲基纤维素钠）和复方鹿角霜低、中、高剂量组（浸膏混悬液6、14、34 g/kg，分别相当于成人临床推荐日剂量的192、448、1 088倍），每组10只，雌雄兼半，均灌胃给药1次。给药前和给药后14 d内，观察小鼠存活情况及其急性毒性反应；给药前、给药时和给药后2、3、7、14 d测定小鼠体质量；给药后14 d解剖小鼠，检查小鼠体表、给药局部和天然孔道，颅腔和脑的外表面，胸腔、腹腔和盆腔以及腔内脏器，记录病变情况。结果：各组小鼠均全部存活。空白对照组和复方鹿角霜低剂量组小鼠未见异常；复方鹿角霜中剂量组雄性小鼠未见明显异常，4只雌性小鼠于给药后20～23 min开始出现眼睛闭合症状，给药后27 min开始逐渐恢复，给药后29 min全部恢复正常；复方鹿角霜高剂量组小鼠于给药后8～29 min开始出现眼睛闭合、活动减少、弓背、竖毛等症状，给药后1 h开始逐渐恢复，给药后6 h全部恢复正常。与空白对照组比较，复方鹿角霜低、中、高剂量组小鼠体质量差异无统计学意义（P＞0.05）。各组小鼠体表、给药局部和天然孔道，颅腔和脑的外表面，胸腔、腹腔和盆腔以及腔内脏器[包括肾上腺（双侧）、脑（大脑、小脑和脑干）、食道、心、肾（双侧）、大肠（盲肠、结肠和直肠）、小肠、肝、肺、胰腺、脾、胸腺、胃、膀胱等]均未见明显病变。结论：在复方鹿角霜灌胃给药34 g/kg剂量下，小鼠产生急性毒性反应；14 g/kg剂量下，部分雌性小鼠产生轻度急性毒性反应；6 g/kg剂量下，未见明显急性毒性反应。 |
| ABSTRACT： | OBJECTIVE： To study the acute toxicity of Compound lujiao cream. METHODS： A total of 40 SPF Kunming mice were divided into blank control group （constant volume of sodium carboxymethylcellulose）， Compound lujiao cream low-dose， medium-dose and high-dose groups （extract suspension， 6， 14， 34 g/kg， as 192， 448， 1 088 folds of recommended human clinical daily dose， respectively）， with 10 mice in each group， male and female in half. They were given medicine intragastrically once.Before medication and within 14 d after medication，the survival， toxic reaction were observed.Body weights of mice were measured before medication， during medication and 2，3，7，14 d after medication. The mice was scarified 14 d after medication. The pathological changes of body surface， administration site and natural channels， the external surface of the cranial cavity and brain， thoracic cavity， abdominal cavity， pelvic cavity and visceral organs were also observed. RESULTS： All mice survived. No abnormality was found in blank control group and Compound lujiao cream low-dose group. In Compound lujiao cream medium-dose group， no significant abnormality was found in male mice； 4 female mice suffered eye closure 20-23 min after medication， and then began to recover 27 min after medication and returned to normal 29 min after medication. In Compound lujiao cream high-dose group， mice suffered from eye closure， activity reduction， arched back and hair shaft 8-29 min after medication， but recovered 1 h after medication. Compared with blank control group， there was no significant difference in body weight of mice in Compound lujiao cream low-dose， medium-dose and high-dose groups （P＞0.05）. There was no significant lesion in body surface， administration site and natural channels， the external surface of the cranial cavity and brain， thoracic cavity， abdominal cavity， pelvic cavity and visceral organs [adrenaline （bilateral）， brain （brain， cerebellum， brain stem）， esophagus， heart， kidney （bilateral）， large intestine （cecum， colon and rectum）， small intestine， liver， lung， pancreas， spleen， thymus， stomach， bladder]. CONCLUSIONS： After using Compound lujiao cream of the intragastric dose of 34 g/kg， the mice suffer from acute toxicity reaction； by the dose of 14 g/kg， some female mice suffer from mild acute toxic reaction； by the dose of 6 g/kg， no obvious acute toxicity reaction was found. |
| 期刊： | 2018年第29卷第14期 |
| 作者： | 罗尚文，王燕燕，张学农，韩师师 |
| AUTHORS： | LUO Shangwen，WANG Yanyan，ZHANG Xuenong，HAN Shishi |
| 关键字： | 复方鹿角霜；小鼠；急性毒性 |
| KEYWORDS： | Compound lujiao cream； Mouse； Acute toxicity |
| 阅读数： | 483 次 |
| 本月下载数： | 3 次 |
* 注：未经本站明确许可，任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作！
