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蝇蛆油对急性皮肤创伤感染模型大鼠愈合的影响及机制研究
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| 篇名： | 蝇蛆油对急性皮肤创伤感染模型大鼠愈合的影响及机制研究 |
| TITLE： | |
| 摘要： | 目的：研究蝇蛆油对急性皮肤创伤感染模型大鼠愈合的影响及机制，为蝇蛆油的进一步开发提供参考。方法：将SD大鼠随机分为正常组、模型组、蝇蛆油组和京万红组（阳性对照），每组70只。除正常组外的其余各组大鼠均通过在伤口处涂抹金黄色葡萄球菌悬液复制急性皮肤创伤感染模型。造模成功后，正常组和模型组大鼠涂抹生理盐水，蝇蛆油组和京万红组大鼠涂抹0.3 mL/100 g的相应药物，每天9：00和17：00各给药1次，连续给药15 d。每天观察各组大鼠创面大体情况并记录创面愈合时间；于给药1、2、4、6、8 d后每组选10只大鼠测定创面组织中羟脯胺酸含量，并测定给药 2、4、6、8 d后创面含水量；末次给药2 h后，每组选10只大鼠测定血清中溶菌酶含量和炎症因子[肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）]水平以及皮肤组织中核转录因子 κB p65（NF-κB p65）（细胞质和细胞核中）和磷酸化NF-κB 抑制因子（p-IκB-α）（细胞质中）蛋白表达。结果：与正常组比较，模型组大鼠创面水肿明显，创面愈合时间为（14.3±2.1） d；给药4、6、8 d后，模型组大鼠创面组织中羟脯氨酸含量显著减少（P＜0.05或P＜0.01）；给药2、4、6、8 d后，模型组大鼠创面含水量显著增加（P＜0.01）；给药15 d后，模型组大鼠血清中溶菌酶、TNF-α、IL-6含量均显著增加（P＜0.01），创面组织中NF-κB p65（细胞质）蛋白表达水平显著降低（P＜0.01），NF-κB p65（细胞核中）和p-IκB-α（细胞核中）蛋白表达水平显著升高（P＜0.01）。与模型组比较，各给药组大鼠上述指标均得到显著改善（P＜0.05或P＜0.01）。结论：蝇蛆油对急性皮肤创伤感染引起的组织损伤具有一定的改善作用，可促进创面愈合；其机制可能是通过促进胶原蛋白生成、增加溶菌酶含量、调节NF-κB信号通路而发挥抗炎作用。 |
| ABSTRACT： | OBJECTIVE： To study the effects of maggot oil on the healing of rat with acute skin trauma and infection and its mechanism， in order and to provide reference for further development of maggot oil. METHODS： SD rats were randomly divided into normal group， model group， maggot oil group and Jingwanhong treatment group （positive control）， with 70 mice in each group. Except for normal group， acute skin trauma and infection model was induced in other groups by smearing Staphylococus aureus suspension at the wound. After modeling， normal group and model group were given normal saline， and maggot oil group and Jingwanhong treatment group were given relevant medicine 0.3 mL/100 g， at 9：00 and 17：00， for consecutive 15 d. Wound and wound healing time of rats were observed in each group. The content of hydroxyproline in wound was determined in 10 rats of each group after 1， 2， 4， 6， 8 d of administration. The content of water in wound was determined after 2， 4， 6， 8 d after administration. 2 h after last administration， the content of lysozyme， the levels of inflammatory factors （TNF-α， IL-6）， the expression of NF-κB p65 （in cytoplasm and nucleus） and p-IκB-α （in cytoplasm） protein were determined in 10 rats of each group. RESULTS： Compared with normal group， wound edema of model group was obvious， and wound healing time was （14.3±2.1） d. After 4， 6， 8 d of medication， the content of hydroxyproline in wound of rats was decreased significantly in model group （P＜0.05 or P＜0.01）. After 2， 4， 6， 8 d of medication， the content of water in wound was increased significantly in model group （P＜0.01）. After 15 d of medication， the serum contents of lysozyme， TNF-α and IL-6 in rats were increased significantly in model group （P＜0.01）. The expression of NF-κB p65 （in cytoplasm） in wound was decreased significantly （P＜0.01）， while the expressions of NF-κB p65 （in nucleus） and p-IκB-α （in nucleus） protein were increased significantly （P＜0.01）. Compared with model group， above indicators of administration groups were improved significantly （P＜0.05 or P＜0.01）. CONCLUSIONS： Maggot oil could protect tissue injury induced by acute skin wound infection， promote wound healing. The possible mechanism might play anti-inflammatory effect through promoting collagen production， increasing lysozyme content， regulating NF-κB signal pathway. |
| 期刊： | 2018年第29卷第11期 |
| 作者： | 韩跃东，张超，张松，张衍国 |
| AUTHORS： | HAN Yuedong，ZHANG Chao，ZHANG Song，ZHANG Yanguo |
| 关键字： | 蝇蛆油；急性皮肤创伤感染模型；愈合；核转录因子κB p65；抗炎作用；大鼠 |
| KEYWORDS： | Maggot oil； Acute skin trauma and infection model； Healing； NF-κB p65； Anti-inflammation effect； Rat |
| 阅读数： | 295 次 |
| 本月下载数： | 3 次 |
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