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藏药小檗皮的醇提工艺优化研究
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| 篇名： | 藏药小檗皮的醇提工艺优化研究 |
| TITLE： | |
| 摘要： | 目的：优化小檗皮的醇提工艺。方法：以小檗皮中木兰花碱、盐酸药根碱、盐酸巴马汀、盐酸小檗碱含量和浸膏量为评价指标，采用均匀设计-综合评分法考察乙醇用量、乙醇体积分数、提取时间对提取工艺的影响；采用3次均匀设计试验分别对第1次、第2次、第3次的提取工艺进行考察，分别确定3次提取试验各自的优化工艺并进行验证试验，计算转移率。结果：最优工艺为取小檗皮粗粉加入15倍量75％乙醇，回流提取2次，每次提取120 min 。验证试验中，小檗皮按优化工艺提取2次后木兰花碱、盐酸药根碱、盐酸巴马汀、盐酸小檗碱的含量分别为58.96、4.82、3.07、23.29 mg/g，转移率分别为93.85％、95.02％、96.28％、94.88％（RSD分别为3.87％、2.64％、4.00％、3.91％，n＝3）。结论：优化的小檗皮乙醇回流提取工艺合理、可行，稳定性好。 |
| ABSTRACT： | OBJECTIVE： To optimize extraction technology of cortex of Berberis dictyophylla by ethanol. METHODS： Using the contents of magnoflorine， jatrorrhizine hydrochloride， palmatine hydrochloride and berberine hydrochloride， the amount of extract as evaluation indexes， the effects of ethanol amount， volume fraction of ethanol and extraction time on extraction technology were investigated by uniform design method-comprehensive scoring method. The extraction methods of first time， second time and third time were investigated by 3 times of uniform design test. The optimal schemes of 3 times of extraction test were determined and validation test was conducted， and the transfer rates were calculated. RESULTS： The optimal technology was as follows as coarse powder of cortex of B. dictyophylla， 15-fold 75％ ethanol， extracting for 2 times， 120 min each time. In validation test， the contents of magnoflorine， jatrorrhizine hydrochloride， palmatine hydrochloride and berberine hydrochloride were 58.96， 4.82， 3.07， 23.29 mg/g after B. dictyophylla was extracted by optimization technology for 2 times. The transfer rates were 93.85％， 95.02％， 96.28％， 94.88％， respectively （RSD＝3.87％， 2.64％， 4.00％， 3.91％， n＝3）. CONCLUSIONS： The optimal ethanol reflux extraction technology of cortex of B. dictyophylla is reasonable and feasible with good stability. |
| 期刊： | 2018年第29卷第7期 |
| 作者： | 郝露，周珍，冯慧，赵娅，周邦华，范刚，赖先荣 |
| AUTHORS： | HAO Lu，ZHOU Zhen，FENG Hui，ZHAO Ya，ZHOU Banghua，FAN Gang，LAI Xianrong |
| 关键字： | 藏药；小檗皮；乙醇提取物；均匀设计法；提取工艺 |
| KEYWORDS： | Tibetan medicine； Cortex of Berberis dictyophylla； Ethanol extract； Uniform design method； Extraction technology |
| 阅读数： | 287 次 |
| 本月下载数： | 5 次 |
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