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HPLC法测定绿芫菁中斑蝥素的含量
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| 篇名： | HPLC法测定绿芫菁中斑蝥素的含量 |
| TITLE： | |
| 摘要： | 目的：建立绿芫菁中斑蝥素的含量测定方法，并将测定结果用于筛选提取斑蝥素来源药材的依据。方法：以丙酮为溶剂，采用超声提取法提取绿芫菁中斑蝥素；采用高效液相色谱法测定斑蝥素的含量；色谱柱为C18，流动相为甲醇-水（23 ∶ 77），检测波长为230 nm，流速为1.0 mL/min，柱温为35 ℃，进样量为10 μL；检测绿芫菁中斑蝥素的含量并与采用《中国药典》方法（以氯仿为提取溶剂）测定含量的结果进行比较。结果：斑蝥素检测质量浓度线性范围为0.2～1.0 mg/mL（r＝0.998 8），平均方法回收率为101.1％（RSD为1.7％，n＝6）；绿芫菁中斑蝥素的平均含量为0.932％（n＝3），采用《中国药典》方法测得的含量为0.793％（n＝3），均高于《中国药典》项下斑蝥素来源药材中斑蝥素含量需大于0.35％的要求。结论：建立的绿芫菁中斑蝥素的含量测定方法准确可靠；绿芫菁中斑蝥素的含量达到《中国药典》的要求，可用作提取斑蝥素的来源药材。 |
| ABSTRACT： | OBJECTIVE： To establish the method for content determination of cantharidin in Lytta caraganae， and to use the result as the extract screening evidence of L. caraganae source material. METHODS： Ultrasonic extraction method was used to extract cantharidin from L. caraganae using acetone as solvent. HPLC method was adopted to determine the content of cantharidin. The determination was performed on C18 column with mobile phase consisted of methanol-water （23 ∶ 77） at flow rate of 1.0 mL/min. The detection wavelength was set 230 nm， the column temperature was set at 35 ℃， and sample size was 10 μL. The content of cantharidin in L. caraganae was determined and compared with the results of content determination by the method stated in Chinese Pharmacopeia （using chloroform as extraction solvent）. RESULTS： The liner range of cantharidin were 0.2-1.0 mg/mL （r＝0.998 8） with average methodology recovery rates of 101.1％ （RSD＝1.7％， n＝6）. The average content of cantharidin in L. caraganae was 0.932％ （n＝3）， while the content of cantharidin was 0.793％ （n＝3） determined by the method stated in Chinese Pharmacopeia. Both were higher than the requirement of Chinese Pharmacopeia that the content of cantharidin in scource material of cantharidin was higher than 0.35％. CONCLUSIONS： Established method is accurate and reliable for the content determination cantharidin in L. caraganae. The content of cantharidin is up to the standard of Chinese Pharmacopeia， and can be used as source material for exacting cantharidin. |
| 期刊： | 2018年第29卷第7期 |
| 作者： | 王腾蛟，赵成坚，谷颖乐，徐永莉，李力 |
| AUTHORS： | WANG Tengjiao，ZHAO Chengjian，GU Yingle，XU Yongli，LI Li |
| 关键字： | 绿芫菁；斑蝥素；高效液相色谱法；丙酮提取；含量测定 |
| KEYWORDS： | Lytta caraganae； Cantharidin；HPLC； Acetone extraction； Content determination |
| 阅读数： | 433 次 |
| 本月下载数： | 6 次 |
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