泽泻汤加味方对高脂血症模型大鼠肝组织中水通道蛋白8的影响-中国药房

泽泻汤加味方对高脂血症模型大鼠肝组织中水通道蛋白8的影响

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篇名：	泽泻汤加味方对高脂血症模型大鼠肝组织中水通道蛋白8的影响
TITLE：
摘要：	目的：观察泽泻汤加味方对高脂血症模型大鼠肝组织中水通道蛋白8（AQP8）表达的影响，探讨该方防治高脂血症的机制。方法：将60只大鼠随机分为空白对照组（蒸馏水）、模型组、阳性对照组（辛伐他汀1.89 mg/kg）和泽泻汤加味方高、中、低剂量组（29.56、14.78、7.39 g/kg，以生药量计），每组10只。除空白对照组大鼠给予正常饮食外，其余各组大鼠均给予高脂饲料以复制高脂血症模型，并于造模同时每天灌胃给药1次，连续给药5周。给药结束后，检测大鼠血清中三酰甘油（TG）、胆固醇（TC）、高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）含量，观察大鼠肝组织病理形态学变化，并检测大鼠肝组织中AQP8 mRNA和蛋白表达水平。结果：与空白对照组比较，模型组大鼠血清中TG、TC、LDL-C含量显著升高（P＜0.05或P＜0.01），血清中HDL-C含量显著降低（P＜0.01），肝组织出现细胞排列不整齐、肝血窦充血水肿等病理学变化，肝组织中AQP8 mRNA和蛋白表达水平显著升高（P＜0.01）；与模型组比较，各给药组大鼠上述指标均显著改善（P＜0.05或P＜0.01），肝组织的结构趋于正常、脂肪变性明显减轻。结论：泽泻汤加味方可能通过下调肝组织中AQP8 mRNA及蛋白的表达，发挥其对高脂血症的防治作用。
ABSTRACT：	OBJECTIVE： To observe the effects of Modified Rhizoma Alismatis decoction on the expression of aquaporin 8 （AQP8） in liver tissue of hyperlipemia model rats， and to investigate the mechanism of preventing and treating hyperlipemia. METHODS： Total of 60 rats were randomly divided into blank control group （distilled water）， model group， positive control group （simvastatin 1.89 mg/kg） and modified Rhizoma Alismatis decoction high-dose， medium-dose and low-dose groups （29.56， 14.78， 7.39 g/kg， calculated by crude drug）， with 10 rats in each group. Those groups were given high-fat diet to induce hyperlipemia model and given relevant medicine intragastrically once a day for consecutive 5 weeks except that blank control group was given normal diet. After administration， the serum contents of TG， TC， HDL-C and LDL-C in rats were detected， and the pathomorphology changes of liver tissue were observed； the mRNA and protein expression of AQP8 in liver tissue were detected. RESULTS： Compared blank control group， the serum contents of TG， TC and LDL-C in model group were increased significantly （P＜0.05 or P＜0.01）， while the serum content of HDL-C was decreased significantly （P＜0.01）； pathological changes were found in liver tissue， such as irregular cell arrangement and hepatic sinusoidal hyperemia and edema； mRNA and protein expression of AQP8 in liver tissue were increased significantly （P＜0.01）. Compared with model group， above indexes of treatment groups were improved significantly （P＜0.05 or P＜0.01）； the structure of liver tissue tended to be normal and the fatty degeneration was obviously alleviated. CONCLUSIONS： Modified Rhizoma Alismatis decoction can regulate the mRNA and protein expression of AQP8 in liver tissue so as to play the effects on the prevention and treatment of hyperlipidemia.
期刊：	2018年第29卷第5期
作者：	张睦清，韩雪，段思明，张一昕，王亚芬，张纳博，王彦蕊
AUTHORS：	ZHANG Muqing，HAN Xue，DUAN Siming，ZHANG Yixin，WANG Yafen，ZHANG Nabo，WANG Yanrui
关键字：	泽泻汤加味方；高脂血症；水通道蛋白8；大鼠
KEYWORDS：	Modified Rhizoma Alismatis decoction； Hyperlipidemia； Aquaporin 8； Rat
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