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枳壳药材挥发油的GC-MS指纹图谱研究
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| 篇名： | 枳壳药材挥发油的GC-MS指纹图谱研究 |
| TITLE： | |
| 摘要： | 目的：建立枳壳药材挥发油的气相色谱-质谱（GC-MS）指纹图谱。方法：采用GC-MS法，色谱柱为RTX-5MS毛细管柱，进样口温度为250 ℃，载气为高纯氦气（≥99.999％），流速为1.0 mL/min，分流比为10 ∶ 1，进样量为1 μL（程序升温）；采用电子轰击离子源，离子源温度为230 ℃，检测器温度为250 ℃，溶剂延迟时间为3 min，扫描范围为m/z 35～550。以月桂烯为参照，测定21批药材挥发油样品的GC-MS图谱，采用《中药色谱指纹图谱相似度评价系统》（2004 A版）进行相似度评价，确定共有峰，采用LC Solution 2质谱库（NIST05.LIB和NIST05s.LIB）确定共有峰成分，并采用面积归一化法测定共有峰的相对含量。结果：21批药材挥发油样品的GC-MS图谱有20个共有峰，相似度均＞0.90；经验证，21批药材挥发油样品GC-MS图谱与对照指纹图谱具有较好的一致性。枳壳药材挥发油主要成分为柠檬烯、萜品烯、月桂烯和D-Cadinene。结论：所建指纹图谱可为枳壳药材挥发油的真伪鉴别和质量评价提供参考。 |
| ABSTRACT： | OBJECTIVE： To establish GC-MS fingerprint of volatile oil from Citrus aurantium. METHODS： GC-MS method was adopted. The determination was performed on RTX-5MS capillary column with injector temperature of 250 ℃， high pure helium as carrier gas（≥99.999％）， flow rate of 1.0 mL/min， split ratio of 10 ∶ 1， and sample size of 1 μL （temperature programming）. Mass spectrum condition included electron bombardment ion source， ion source temperature of 230 ℃， detector temperature of 250 ℃， 3 min solvent delay， scanning range of m/z 35-550. GC-MS chromatograms of 21 batches of volatile oil samples were determined using Laurene as reference. The similarity of them was evaluated by using TCM Chromatographic Fingerprint Similarity Evaluation System （2004 A edition）， and common peak was determined. The components of common peak were determined by LC Solution 2 mass database （NIST05.LIB and NIST05s.LIB）. Relative content of common peak was determined with area normalization. RESULTS： There were 20 common peaks in GC-MS chromatograms of 21 batches of volatile oil samples， and the similarity was higher than 0.90. After validation， GC-MS chromatograms of 21 batches of volatile oil samples were in good agreement with control fingerprint. The main constituents of the volatile oil of C. aurantium were Limonene，Terpinene， Laurene and D-Cadinene. CONCLUSIONS： Established fingerprint can provide reference for identification and quality evaluation of volatile oil of C. aurantium. |
| 期刊： | 2018年第29卷第4期 |
| 作者： | 刘清茹，谭伟民，文诗泳，肖幸华，陈婷，郭英，曾晓艳，刘塔斯 |
| AUTHORS： | LIU Qingru，TAN Weimin，WEN Shiyong，XIAO Xinghua，CHEN Ting，GUO Ying，ZENG Xiaoyan，LIU Tasi |
| 关键字： | 枳壳；挥发油；气相色谱-质谱指纹图谱；道地药材 |
| KEYWORDS： | Citrus aurantium； Volatile oil； GC-MS fingerprint； Genuine regional drug |
| 阅读数： | 452 次 |
| 本月下载数： | 13 次 |
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