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苗药透骨香的质量标准研究
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| 篇名： | 苗药透骨香的质量标准研究 |
| TITLE： | |
| 摘要： | 目的：建立透骨香药材的质量标准。方法：采用薄层色谱法（TLC）对药材进行定性鉴别；测定药材水分、总灰分和酸不溶性灰分；采用高效液相色谱法测定药材中水杨酸甲酯的含量：色谱柱为Eclipse XDB-C18，流动相为甲醇-水（62 ∶ 38，V/V），流速为1.0 mL/min，检测波长为307 nm，柱温为30 ℃，进样量为10 μL。结果：药材的TLC图斑点清晰，分离度好。药材水分为8.2％～10.8％，总灰分为0.9％～4.0％，酸不溶性灰分为0.1％～0.9％。水杨酸甲酯检测进样量线性范围为0.045～0.73 μg（r＝0.999 9）；精密度、稳定性、重复性试验的RSD≤1.0％；水杨酸甲酯加样回收率为97.8％～104.3％（RSD＝2.6％，n＝9）。结论：该研究所建标准可用于透骨香药材的质量控制。 |
| ABSTRACT： | OBJECTIVE： To establish the quality standard for Gaultheria yunnanensis. METHODS： TLC was adopted for qualitative identification of samples. Moisture， total ash and acid-insoluble ash were determined. HPLC method was used to determine the content of methyl salicylate. The determination was performed on Eclipse XDB-C18 column with mobile phase consisted of methanol-water（62 ∶ 38，V/V）at the flow rate of 1.0 mL/min. The detection wavelength was set at 307 nm， and column temperature was 30 ℃. The sample size was 10 μL. RESULTS： TLC spots of samples were clear and well separated. The moisture was 8.2％-10.8％， total ash was 0.9％-4.0％ and acid-insoluble ash was 0.1％-0.9％. The linear range of methyl salicylate were 0.045-0.73 μg （r＝0.999 9）. RSDs of precision， stability and reproducibility tests were no more than 1.0％. The recoveries of methyl salicylate were 97.8％-104.3％ （RSD＝2.6％，n＝9）. CONCLUSIONS： The established standard can be used for quality control of G. yunnanensis. |
| 期刊： | 2017年第28卷第33期 |
| 作者： | 姚成芬，张宝，熊丹丹，刘亭，郑林，李月婷，陈青凤，李勇军 |
| AUTHORS： | YAO Chengfen，ZHANG Bao，XIONG Dandan，LIU Ting，ZHENG Lin，LI Yueting，CHEN Qingfeng，LI Yongjun |
| 关键字： | 透骨香；薄层色谱法；高效液相色谱法；质量标准； 滇白珠苷A；水杨酸甲酯 |
| KEYWORDS： | Gaultheria yunnanensis； TLC； HPLC； Quality standard； Gaultheroside A； Methyl salicylate |
| 阅读数： | 377 次 |
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