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贞芪扶正颗粒的HPLC指纹图谱研究
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| 篇名： | 贞芪扶正颗粒的HPLC指纹图谱研究 |
| TITLE： | |
| 摘要： | 目的：建立贞芪扶正颗粒的高效液相色谱（HPLC）指纹图谱。方法：采用高效液相色谱-蒸发光散射检测法（HPLC-ELSD）。色谱柱为Agilent Zorbax Eclipse XDB-C18，流动相为乙腈-水（梯度洗脱），流速为1.0 mL/min，柱温为40 ℃，检测器蒸发光温度为50 ℃，进样量为10 μL。以特女贞苷为参照，测定7批贞芪扶正颗粒的HPLC图谱，采用《中药色谱指纹图谱相似度评价系统》（2004 A）版进行共有峰指认和相似度评价。结果：7批贞芪扶正颗粒的HPLC图谱有13个共有峰。经验证，7批样品HPLC图谱中有3批样品相似度＞0.9，与对照指纹图谱具有较好一致性；4批样品相似度＜0.9，与对照指纹图谱一致性较差。结论：该研究所建指纹图谱可为贞芪扶正颗粒的质量评价提供参考。 |
| ABSTRACT： | OBJECTIVE： To establish HPLC fingerprint of Zhenqi fuzheng granules. METHODS： HPLC-ELSD method was adopted. The determination was performed on Agilent Zorbax Eclipse XDB-C18 column with mobile phase consisted of acetonitrile-water （gradient elution） at the flow rate of 1.0 mL/min with column temperature of 40 ℃. The detector evaporation temperature was 50 ℃， and sample size was 10 μL. Using specnuezhenide as reference， HPLC chromatograms of 7 batches of Zhenqi fuzheng granules were determined. The identification and similarity evaluation of common peaks were conducted by using the TCM Chromatographic Fingerprint Similarity Evaluation System （2004 A edition）. RESULTS： There were 13 common peaks in HPLC chromatograms of 7 batches of samples. After validated， the similarity of 3 batches of samples in HPLC chromatograms of 7 batches of samples were higher than 0.9， which were in good agreement with control fingerprints. The similarity of 4 batches of samples were ＜0.9，which were poorly same to control fingerprint. CONCLUSIONS： Established fingerprint can provide reference for quality evaluation of Zhenqi fuzheng granules. |
| 期刊： | 2017年第28卷第33期 |
| 作者： | 魏晓雨，姜国栋，陈泓，王美慧，徐杰，边雨，姜文月 |
| AUTHORS： | WEI Xiaoyu，JIANG Guodong，CHEN Hong，WANG Meihui，XU Jie，BIAN Yu，JIANG Wenyue |
| 关键字： | 贞芪扶正颗粒；高效液相色谱-蒸发光散射检测法；指纹图谱；质量标准 |
| KEYWORDS： | Zhenqi?fuzheng?granules； HPLC-ELSD； Fingerprint； Quality standard |
| 阅读数： | 360 次 |
| 本月下载数： | 2 次 |
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