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高效液相色谱法测定人血浆中伏立康唑的浓度
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| 篇名： | 高效液相色谱法测定人血浆中伏立康唑的浓度 |
| TITLE： | |
| 摘要： | 目的：建立测定人血浆中伏立康唑浓度的方法。方法：血浆样品经乙腈沉淀蛋白后，以酮康唑为内标，采用高效液相色谱法测定。色谱柱为Dionex U-3000 Dimonsil C18，流动相为三乙胺-冰醋酸-水混合溶液（1 ∶ 1 ∶ 98，V/V/V，pH约为4.0）-乙腈（40 ∶ 60，V/V），流速为1.0 mL/min，检测波长为255 nm，柱温为40 ℃，进样量为20 μL。结果：伏立康唑血药浓度在0.2～20.0 μg/mL范围内线性关系良好，定量下限为0.2 μg/mL，最低检测限为0.03 μg/mL；日内、日间RSD均小于10％；方法回收率为92.06％～106.26％（RSD＜5％，n＝5），提取回收率为75.62％～90.59％（RSD＜5％，n＝5）。采用该法测得10例患儿体内伏立康唑的血药浓度为0.22～4.90 μg/mL（n＝10）。结论：该方法简单、快速、专属性强，可用于伏立康唑治疗药物监测。 |
| ABSTRACT： | OBJECTIVE： To establish a method for the concentration determination of voriconazole in human plasma. METHODS： Plasma samples were precipitated with acetonitrile. Using ketoconazole as internal standard， HPLC method was adopted. The determination was performed on Dionex U-3000 Dimonsil C18 column with mobile phase consisted of triethylamine-glacial acetic acid-water mixed solution （1 ∶ 1 ∶ 98，V/V/V， pH was about 4.0）-acetonitrile （40 ∶ 60，V/V） at the flow rate of 1.0 mL/min. The detection wavelength was set at 255 nm. The column temperature was 40 ℃， and sample size was 20 μL. RESULTS： The linear range of voriconazole was 0.2-20.0 μg/mL. The limits of quantification was 0.2 μg/mL， and the minimum detection limit was 0.03 μg/mL. RSDs of inter-day and intra-day were lower than 10％. The method recoveries were 92.06％-106.26％（RSD＜5％，n＝5）， and extraction recoveries were 75.62％-90.59％（RSD＜5％，n＝5）. The plasma concentration of voriconazole in 10 children ranged 0.22-4.90 μg/mL （n＝10）. CONCLUSIONS： The method is simple， rapid， specific and can be used for drug monitoring of voriconazole. |
| 期刊： | 2017年第28卷第32期 |
| 作者： | 李学娟，刘四喜，陈泽彬，魏红，杨卫国，温鹏强 |
| AUTHORS： | LI Xuejuan，LIU Sixi，CHEN Zebin，WEI Hong，YANG Weiguo，WEN Pengqiang |
| 关键字： | 伏立康唑；血药浓度；高效液相色谱法 |
| KEYWORDS： | Voriconazole； Plasma concentration； HPLC |
| 阅读数： | 532 次 |
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