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蒙药五味沙棘散对吸烟致小鼠肺部炎症的改善作用及机制研究
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| 篇名： | 蒙药五味沙棘散对吸烟致小鼠肺部炎症的改善作用及机制研究 |
| TITLE： | |
| 摘要： | 目的：研究蒙药五味沙棘散（ WSP）对吸烟所致小鼠肺部炎症的改善作用及其机制。方法：将30只ICR小鼠随机分为空白组（生理盐水）、模型组（生理盐水）和WSP组（2 g/kg）。模型组和WSP组小鼠采用被动吸烟法复制肺部炎症损伤模型，并于造模的同时每天ig相应药物1次，连续28 d。给药结束后，采用酶联免疫吸附法检测小鼠肺泡灌洗液（BALF）中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、IL-6和IL-10水平；苏木精-伊红染色后光镜下观察小鼠肺组织病理变化；Western blot法检测小鼠肺组织中细胞外信号调节蛋白激酶1/2（ERK1/2）、磷酸化ERK1/2（p-ERK1/2）、p38丝裂原活化蛋白激酶（p38 MAPK）、磷酸化p38 MAPK（p-p38 MAPK）、核因子κB p65（NF-κB p65）和磷酸化NF-κB p65（p-NF-κB p65）蛋白表达。结果：与空白组比较，模型组小鼠BALF中TNF-α、IL-1β和IL-6水平明显升高（P＜0.01），肺组织发生明显炎症病变，肺组织中p-ERK1/2、p-p38 MAPK、p-NF-κB p65蛋白表达水平明显升高（P＜0.01）。与模型组比较，WSP组大鼠BALF中TNF-α、IL-1β水平明显降低（P＜0.05），肺组织炎症损伤明显改善，肺组织中p-p38 MAPK和p-NF-κB p65蛋白表达水平明显降低（P＜0.05）。结论：WSP可能通过阻断p38 MAPK、NF-κB p65蛋白的磷酸化来抑制炎症因子TNF-α和IL-6的高表达，从而发挥其对吸烟所致小鼠肺部炎症损伤的改善作用。 |
| ABSTRACT： | OBJECTIVE： To study the improvement effect and mechanism of Meng medicine Wuwei Shaji powder （WSP） on smoke-induced lung inflammation injury in mice. METHODS： ICR mice were randomly divided into blank group （normal saline）， model group （normal saline） and WSP group （2 g/kg）. Mice in model group and WSP group received passive smoking to induce model of lung inflammation injury， and intragastrically administrated relevant medicine when modeling， once a day， for 28 d. After administration， enzyme-linked immunosorbent assay was used to detect the tumor necrosis factor α （TNF-α）， interleukin-1β （IL-1β）， IL-6， IL-10 levels in bronchoalveolar lavage fluids （BALF）； the pathological changes of lung tissue were observed by optical microscope after hematoxylin-eosin staining； Western blot was adopted to detect the protein expressions of extracellular signal-regulated kinase （ERK1/2）， phosphorylated ERK1/2 （p-ERK1/2）， p38 mitogen-activated protein kinase （p38 MAPK）， phosphorylated p38 MAPK （p-p38 MAPK）， nuclear factor kappaB p65 （NF-κB p65） and phosphorylated NF-κB p65 （p-NF-κB p65） in lung tissue of mice. RESULTS： Compared with blank group， TNF-α， IL-1β， IL-6 levels in BALF in model group were obviously increased （P＜0.01）； lung tissue showed significant inflammatory lesions； and the protein expressions of p-ERK1/2， p-p38 MAPK， p-NF-κB p65 in lung tissue were obviously increased （P＜0.01）. Compared with model group， TNF-α， IL-1β levels in BALF in WSP group were obviously decreased （P＜0.05）； inflammation injury in lung tissue was obviously improved； and protein expressions of p-p38 MAPK and p-NF-κB p65 in lung tissue were obviously decreased （P＜0.05）. CONCLUSIONS： WSP shows improvement effect on smoke-induced lung inflammation injury in mice， which might be by blocking the p38 MAPK， NF-κB p65 phosphorycation to inhibit the high expression of inflammatory factors as TNF-α and IL-6. |
| 期刊： | 2017年第28卷第31期 |
| 作者： | 包桂兰，王秀枝，梁凤娟，刘佳，刘铮 |
| AUTHORS： | BAO Guilan，WANG Xiuzhi，LIANG Fengjuan，LIU Jia，LIU Zheng |
| 关键字： | 蒙药；五味沙棘散；吸烟；炎症；小鼠 |
| KEYWORDS： | Meng medicine； Wuwei Shaji powder； Smoke； Inflammation； Mice |
| 阅读数： | 288 次 |
| 本月下载数： | 0 次 |
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