Box-Behnken响应面法优化水溶性壳聚糖对黄莪凝胶水提液的除杂工艺
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篇名: Box-Behnken响应面法优化水溶性壳聚糖对黄莪凝胶水提液的除杂工艺
TITLE:
摘要: 目的:优化水溶性壳聚糖对黄莪凝胶水提液除杂的工艺条件。方法:以透光率比、杂质去除率、柚皮苷保留率的综合评分为指标,采用Box-Behnken响应面法优化除杂工艺中水溶性壳聚糖用量、振荡时间和温度,并进行验证。结果:比重≥1.3的中药水提液与0.01 g/mL水溶性壳聚糖溶液用量的体积比为2.2,70 ℃下振荡100 min除杂效果最佳;验证试验中3批样品的平均透光率比为87.3%、杂质去除率为41.50%、柚皮苷保留率为131.38%,综合评分的实测值分别为97.35%、98.92%、98.04%,与预测值(98.27%)的相对误差分别为-0.87%、0.73%、-0.16%。结论:该方法可有效优化水溶性壳聚糖对黄莪凝胶水提液的除杂工艺参数。
ABSTRACT: OBJECTIVE: To optimize the technology conditions of water-soluble chitosan on Huang’e gel water extract solution. METHODS: Using the comprehensive scores of transmittance ratio, impurity removal rate, naringin retention rate as indexes, Box-Behnken response surface method was used to optimize the amount of water-soluble chitosan, shocking time and temperature in clarifying technology, and verification test was conducted. RESULTS: The clarification effect was the best when the volume ratio of TCM extract solution with proportion ≥1.3 to amount of 0.01 g/mL water-soluble chitosan was 2.2, shocking for 100 min at 70 ℃. In the verification test, the average transmittance ratio was 87.3%, impurity removal rate was 41.5%, and naringin retention rate was 131.38%. The measured values of comprehensive scores were 97.35%, 98.92% and 98.04%, showing relative error of -0.87%, 0.73% and -0.16% with the predicted values (98.27%), respectively. CONCLUSIONS: The method can effectively optimize the clarification technology parameters of water-soluble chitosan on Huang’e gel water extract solution.
期刊: 2017年第28卷第28期
作者: 王冬东,张辉靠,孙程,吕晓敏,胡荣
AUTHORS: WANG Dongdong,ZHANG Huikao,SUN Cheng,LYU Xiaomin,HU Rong
关键字: 响应面法;水溶性壳聚糖;中药水提液;柚皮苷
KEYWORDS: Response?surface?method;?Water-soluble?chitosan;?TCM?extract solution; Naringin
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