响应面法优化栀子中总多酚、总黄酮的提取工艺
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篇名: 响应面法优化栀子中总多酚、总黄酮的提取工艺
TITLE:
摘要: 目的:优化栀子中总多酚、总黄酮的提取工艺。方法:通过Plackett-burman(PB)设计筛选乙醇体积分数、液料比、药材粒径、提取时间、提取温度5个因素中对栀子总多酚、总黄酮提取的关键影响因素,再采用星点设计结合响应面法对提取工艺中关键影响因素进行优化,并进行验证试验。结果:栀子总多酚的最优提取条件为40%乙醇、药材粒径0.20 mm、提取温度60 ℃、液料比20倍、提取时间20 min;总黄酮的最优提取条件为40%乙醇、药材粒径0.20 mm、提取温度30 ℃、液料比20倍、提取时间20 min。验证试验中栀子总多酚、总黄酮的含量分别为1.70%(RSD=1.43%,n=3)、3.23%(RSD=3.72%,n=3),与预测值的相对误差分别为1.80%、8.75%。结论:采用基于PB和星点设计的响应面法优化栀子总多酚、总黄酮的提取工艺简便、合理、可行,本方法可为其工业化提取提供参考。
ABSTRACT: OBJECTIVE: To optimize the extraction technology of total polyphenol and total flavonoid in Gardenia jasminoides. METHODS: Plackett-burman (PB) design was used to select the ethanol volume fraction, liquid-solid ratio, particle size, extraction time and extraction temperature to determine the key factor affecting the extraction of total polyphenol and total flavonoids in G. jasminoides. Then central composite design (CCD) was combined with response surface method to optimize the extraction technology, and verification test was conducted. RESULTS: The optimal extraction conditions of total polyphenol were 40% ethanol, particle size of 0.20 mm, extraction temperature of 60 ℃, liquid-solid ratio of 20, and extraction time of 20 min; the optimal extraction conditions of total flavonoids were 40% ethanol, particle size of 0.20 mm, extraction temperature of 30 ℃, liquid-solid ratio of 20, and extraction time of 20 min. In verification test, the contents of total polyphenol and total flavonoids in G. jasminoides were 1.70% (RSD=1.43%, n=3), 3.23% (RSD=3.72%, n=3), with relative error of 1.80%, 8.75% with predicted values, respectively. CONCLUSIONS: The response surface method based on PB and CCD is simple, reasonable and feasible to optimize the extraction technology of total polyphenol and total flavonoids in G. jasminoides. The method can provide reference for its industrial extraction.
期刊: 2017年第28卷第28期
作者: 黄潇,刘婧,彭水梅,刘德鸿,付小梅,裴建国,吴志瑰
AUTHORS: HUANG Xiao,LIU Jing,PENG Shuimei,LIU Dehong,FU Xiaomei,PEI Jianguo,WU Zhigui
关键字: 栀子;多酚;黄酮;Plackett-burman设计;星点设计;响应面法;提取工艺;优化
KEYWORDS: Gardenia jasminoides; Polyphenol; Flavonoid; Plackett-burman design; Central composite design; Response surface method; Extraction technology; Optimization
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