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丹酚酸B抗心肌纤维化的机制研究
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| 篇名： | 丹酚酸B抗心肌纤维化的机制研究 |
| TITLE： | |
| 摘要： | 目的：研究丹酚酸B（Sal B）对血管紧张素Ⅱ（Ang Ⅱ）诱导的心肌成纤维细胞增殖、Ⅲ型胶原分泌及基质金属蛋白酶9（MMP-9）、Smad2/3、Smad7蛋白表达的影响，探讨其抗心肌纤维化的作用机制。方法：将细胞分为空白对照组（培养液）、AngⅡ模型组和Sal B低、中、高浓度组（12.5、25、50 μmol/L），用空白或含药培养液培养细胞1 h后，除空白对照组外的其余各组均加入1 μmol/L的AngⅡ诱导细胞增殖，共同作用24 h。分别采用MTT法和苏木精-伊红染色法考察Sal B对细胞增殖的影响；采用Western blot法检测Sal B对细胞Ⅲ型胶原、MMP-9 、Smad2/3、Smad7蛋白表达的影响。结果：与空白对照组比较，AngⅡ模型组细胞明显增殖，Ⅲ型胶原、MMP-9、Smad2/3蛋白表达明显增强，Smad7蛋白表达明显减弱，差异均有统计学意义（P＜0.05）；与AngⅡ模型组比较，Sal B各浓度组细胞的增殖均受到抑制，Ⅲ型胶原、MMP-9、Smad2/3蛋白表达均减弱，Smad7蛋白表达均增强，除Sal B低、中浓度组细胞Ⅲ型胶原与Sal B高浓度组细胞Smad2/3蛋白表达变化不显著外，其余各指标差异均有统计学意义（P＜0.05）。结论：Sal B抗心肌纤维化的作用可能与抑制心肌成纤维细胞的增殖，下调Ⅲ型胶原、MMP-9、Smad2/3蛋白表达和上调Smad7蛋白表达有关。 |
| ABSTRACT： | OBJECTIVE： To study the effects of salvianolic acid B （Sal B） on angiotensin Ⅱ （Ang Ⅱ）-induced cardiac fibroblast proliferation， secretion of type Ⅲ collagen， protein expressions of matrix metalloproteinase 9 （MMP-9）， Smad2/3， Smad7， and explore its mechanism of anti-myocardial fibrosis. METHODS： Cells were divided into blank control group （culture medium） Ang Ⅱ model group， Sal B low-dose， medium-dose， high-dose groups （12.5， 25， 50 μmol/L）. After cultured 1 h by blank or drug-containing culture， except for blank control group， cells in other groups were added 1 μmol/L Ang Ⅱ to induce proliferation. for 24 h. MTT method and hematoxylin-eosin staining method were adopted investigate the effect of Sal B on proliferation. Western blot method was adopted to detect the effects of Sal B on protein expressions of type Ⅲ collagen， MMP-9， Smad2/3， Smad7. RESULTS： Compared with blank control group， cells in Ang Ⅱ model group were significantly proliferated， protein expressions of type Ⅲ collagen， MMP-9， Smad2/3 were obviously enhanced， protein expression of Smad7 was obviously weakened， with statistical significances （P＜0.05）. Compared with Ang Ⅱ model group， the cell proliferation in Sal B groups were inhibited， protein expressions of type Ⅲ collagen， MMP-9， Smad2/3 were weakened， while protein expression of Smad7 was enhanced. Except the protein expression of type Ⅲ collagen in Sal B low-dose and medium-dose groups， the protein expression of Smad2/3 in Sal B high-dose group did not change significantly， other indexes had statistical significances （P＜0.05）. CONCLUSIONS： The anti-myocardial fibrosis effect of Sal B may be associated with inhibiting the proliferation of cardiac fibroblasts， down-regulating protein expressions of type Ⅲ collagen， MMP-9， Smad2/3 and up-regulating protein expression of Smad7. |
| 期刊： | 2017年第28卷第28期 |
| 作者： | 罗红，王春花，赵玲璐，杨宇，陈世平，徐旖旎，杨红宇，沈祥春 |
| AUTHORS： | LUO Hong，WANG Chunhua，ZHAO Linglu，YANG Yu，CHEN Shiping，XU Yini，YANG Hongyu，SHEN Xiangchun |
| 关键字： | 丹酚酸B；心肌成纤维细胞；Ⅲ型胶原；基质金属蛋白酶9；转化生长因子β1信号通路 |
| KEYWORDS： | Salvianolic acid B； Cardiac fibroblasts； Type Ⅲ collagen； Matrix metalloproteinase 9； Transforming growth factor β1 signaling pathway |
| 阅读数： | 366 次 |
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