广西地不容药材的HPLC指纹图谱研究-中国药房

广西地不容药材的HPLC指纹图谱研究

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篇名：	广西地不容药材的HPLC指纹图谱研究
TITLE：
摘要：	目的：建立广西地不容药材的高效液相色谱（HPLC）指纹图谱。方法：采用HPLC法。色谱柱为Hypersil ODS2，流动相为乙腈-0.5％磷酸溶液（梯度洗脱），流速为1.0 mL/min，检测波长为282 nm，柱温为30 ℃，进样量为10 μL。以延胡索乙素为参照，测定10批药材样品的HPLC图谱，采用《中药色谱指纹图谱相似度评价系统》（2012 版）进行共有峰指认和相似度评价。结果：10批广西地不容的HPLC图谱有17个共有峰，10批药材样品中有8个产地药材指纹图谱与对照图谱比较相似度＞0.900，2个产地药材相似度＜0.900。结论：该研究所建HPLC指纹图谱可为广西地不容的鉴别和质量评价提供参考；广西区内多数产地的地不容药材所含生物碱类成分相似，少数存在差异。
ABSTRACT：	OBJECTIVE： To establish HPLC fingerprints of Stephania kwangsiensis. METHODS： HPLC method was adopted. The determination was performed on Hypersil ODS2 with mobile phase consisted of acetonitrile -0.5％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 282 nm， and column temperature was 30 ℃. The sample size was 10 μL. Using tetrahydropalmatine as reference， HPLC fingerprints of 10 batches of medicinal materials were determined. Common peak identification and similarity evaluation were conducted by using Similarity Evaluation System of TCM Chromatographic Fingerprint （2012 edition）. RESULTS： A total of 17 common peaks were identified in HPLC fingerprints of 10 batches of S. kwangsiensis. Among 10 batches of samples， fingerprint of samples from 8 producing areas were compared with control chromatogram. The similarity was higher than 0.900. The similaritg of samples from 2 producing areas were lower than 0.900. CONCLUSIONS： Established HPLC fingerprint can provide reference for the identification and quality evaluation of S. kwangsiensis； S. kwangsiensis in Guangxi from most producing areas include similar alkaloid components，but samples from other producing areas are different from them.
期刊：	2017年第28卷第27期
作者：	黄秋洁，叶勇，王捷，王志萍，韦炜，李丹，朱滴新
AUTHORS：	HUANG Qiujie，YE Yong，WANG Jie，WANG Zhiping，WEI Wei，LI Dan，ZHU Dixin
关键字：	广西地不容；高效液相色谱法；指纹图谱；相似度
KEYWORDS：	Stephania kwangsiensis in Guangxi； HPLC； Fingerprint； Similarity
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