莪术单煎及与三棱合煎后挥发油中有效成分煎出率的比较研究
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篇名: 莪术单煎及与三棱合煎后挥发油中有效成分煎出率的比较研究
TITLE:
摘要: 目的:研究莪术与三棱配伍前后莪术挥发油中4种有效成分的煎出率变化,为揭示莪术与三棱配伍的作用机制提供参考。方法:采用高效液相色谱法同时测定莪术单煎及与三棱合煎后挥发油中4种有效成分(莪术二酮、莪术醇、吉马酮、β-榄香烯)的含量,并对各成分的煎出率进行比较。结果:与莪术单煎比较,合煎后挥发油中莪术二酮、莪术醇、吉马酮的煎出量差异具有统计学意义(P<0.05),β-榄香烯的煎出率差异无统计学意义(P>0.05)。结论:莪术与三棱配伍合煎后可有效增加其挥发油中有效成分莪术二酮、莪术醇和吉马酮的溶出,推测此可能为二者配伍后药效增强的原因 。
ABSTRACT: OBJECTIVE: To study the decoction rate changes of 4 active ingredients in the volatile oil of Curcuma phaeocaulis before and after combined with Sparganium stoloniferum, and provide reference for showing the compatibility mechanism of C. phaeocaulis and S. stoloniferum. METHODS: HPLC was used to simultaneously determine the contents of 4 active ingredients (curdione, curcumol, germacrone and β-elemene) in the volatile oil of C. phaeocaulis after C. phaeocaulis single decoction and combined decoction with S. stoloniferum. And the fried rates of each ingredient were compared. RESULTS: Compared with C. phaeocaulis single decoction, there was significant difference in the fried amounts of curdione, curcumol, germacrone in the volatile oil after combined decoction (P<0.05) and no significant difference in fried rate of β-elemene (P>0.05). CONCLUSIONS: The combined decoction of C. phaeocaulis and S. stoloniferum can effectively improve the dissolution of curdione, curcumol, germacrone in the volatile oil. It may be the reason for the enhancement of efficacy after compatibility.
期刊: 2017年第28卷第22期
作者: 李文静,崔涛,蔡德富,樊丽,张昊,刘雷,洪博
AUTHORS: LI Wenjing,CUI Tao,CAI Defu,FAN Li,ZHANG Hao,LIU Lei,HONG Bo
关键字: 莪术;三棱;合煎;配伍;高效液相色谱法;煎出率
KEYWORDS: Curcuma phaeocaulis; Sparganium stoloniferum; Combined decoction; Compatibility; HPLC; Fried rate
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