GC-MS法分析红木香药材根和果实中的挥发油成分
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篇名: GC-MS法分析红木香药材根和果实中的挥发油成分
TITLE:
摘要: 目的:分析红木香药材根和果实中的挥发油成分。方法:采用气相色谱-质谱联用法。色谱条件:色谱柱为HP-5MS石英弹性毛细管柱,载气为高纯氮气,流速为0.9 mL/min,进样口温度为250 ℃,色谱柱初始温度为50  ℃(程序升温),柱压为80 kPa,分流进样,分流比为40 ∶ 1,进样量为1.0 μL。质谱条件:离子源为电子轰击离子源,电子能量为70 eV,接口温度为230 ℃,质量扫描范围m/z 40~400,扫描间歇时间为1.0 s。结果:从红木香根的挥发油中总共检出43个色谱峰,鉴定出其中24个,占总量的91.5%;主要成分为倍半萜和单萜类化合物,其中 (+)-δ-杜松烯、γ-毕澄茄烯和异朱栾倍半萜相对含量较高。从红木香果实的挥发油中总共检出52个色谱峰,鉴定出其中36个,占总量的82.2%;主要成分为倍半萜和单萜类化合物,其中石竹烯、γ-毕澄茄烯和紫穗槐烯相对含量较高。结论:该研究基本明确了红木香药材根和果实挥发油中的主要成分,并且二者存在明显差异。
ABSTRACT: OBJECTIVE: To analyze volatile oil from the roots and fruits of Kadsura longipedunculata. METHODS: GC-MS method was adopted. The determination was performed on HP-5MS quartz capillary column with nitrogen as carrier gas at the flow rate of 0.9 mL/min. The temperature of sample inlet was 250 ℃, the initial temperature of column was set at 50 ℃ (temperature programming). The column pressure was 80 kPa; the ratio of split sampling was 40 ∶ 1. The sample size was 1.0 μL. Mass chromatography was as follows: electron bombardment ion source as ion source; electron energy of 70 eV; inlet temperature was 230 ℃; scanning range of m/z 40-400, scanning interval time of 1.0 s. RESULTS: Totally 43 chromatographic peaks were detected in volatile oil from the roots of K. longipedunculata, and 24 peaks were identified, accounting for 91.5%; main components were sesquiterpenes and monoterpenoids, among which the relative contents of (+)-delta-cadinene,γ-cadinene and isovalencene were in high level. Totally 52 chromatographic peaks were detected in volatile oil from the fruits of K. longipedunculata, and 36 peaks were identified, accounting for 82.2%; main components were sesquiterpenes and monoterpenoids, among which relative contents of carophyllene, γ-cadinene and valencene were in high level. CONCLUSIONS: The study basically confirm main volatile components in volatile oil from the roots and fruits of K. longipedunculata, and the roots and fruits of K. longipedunculata have significant difference.
期刊: 2017年第28卷第21期
作者: 姜泽静,黄泽豪
AUTHORS: JIANG Zejing,HUANG Zehao
关键字: 红木香;挥发油;气相色谱-质谱联用法
KEYWORDS: Kadsura longipedunculata; Volatile oil; GC-MS
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