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葛花配方颗粒的UPLC指纹图谱研究
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| 篇名： | 葛花配方颗粒的UPLC指纹图谱研究 |
| TITLE： | |
| 摘要： | 目的：建立葛花配方颗粒的超高效液相色谱（UPLC）指纹图谱。方法：采用UPLC法。色谱柱为Zorbax Eclipse XDB-C18，流动相为乙腈-水（梯度洗脱），流速为0.5 mL/min，检测波长为264 nm，柱温为25 ℃，进样体积为1 μL。以鸢尾苷元为参照物，测定10批葛花配方颗粒的UPLC图谱，采用《中药色谱指纹图谱相似度评价系统》（2004 A版）进行共有峰指认和相似度评价。结果：10批葛花配方颗粒的UPLC图谱有14个共有峰，相似度均＞0.90。经验证，10批样品UPLC图谱与对照指纹图谱具有较好的一致性。结论：该研究所建UPLC指纹图谱可为葛花配方颗粒的鉴别和质量评价提供参考。 |
| ABSTRACT： | OBJECTIVE： To establish UPLC fingerprint of Gehua formula granules. METHODS： UPLC method were adopted. The determination was performed on Zorbax Eclipse XDB-C18 column with mobile phase consisted of acetonitrile-water at the flow rate of 0.5 mL/min. The detection wavelength was set at 264 nm， column temperature was 25 ℃， and the sample size was 1 μL. Using tectorigenin as reference substance， UPLC chromatograms of 10 batches of Gehua formula granules were determined. The common peak identification and similarity evaluation were conducted by TCM Chromatogram Fingerprint Similarity Evaluation System （2004 A edition）. RESULTS： 14 common peaks were identified in UPLC chromatograms of 10 batches of Gehua formula granules and similarities were all higher than 0.90. UPLC chromatograms of 10 batches of samples were in good agreement with control fingerprint. CONCLUSIONS： Established UPLC fingerprint can provide reference for identification and quality evaluation of Gehua formula granules. |
| 期刊： | 2017年第28卷第21期 |
| 作者： | 刘梦云，江洁怡，毕晓黎，李素梅，李养学 |
| AUTHORS： | LIU Mengyun，JIANG Jieyi，BI Xiaoli，LI Sumei，LI Yangxue |
| 关键字： | 葛花配方颗粒；超高液相色谱法；指纹图谱；鸢尾苷元 |
| KEYWORDS： | Gehua formula granules； UPLC； Fingerprint； Tectorigenin |
| 阅读数： | 280 次 |
| 本月下载数： | 6 次 |
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