正交试验优化独子藤果实中雷公藤红素的醇提工艺
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篇名: 正交试验优化独子藤果实中雷公藤红素的醇提工艺
TITLE:
摘要: 目的:优选独子藤果实中雷公藤红素的醇提工艺。方法:采用高效液相色谱法测定雷公藤红素的含量。以提取时间、提取次数、料液比为考察因素,以雷公藤红素的提取率为考察指标设计正交试验,并进行验证试验。结果:最优醇提工艺为45%甲醇提取3次、每次2 h、料液比1 ∶ 8;验证试验中,雷公藤红素的平均提取率为0.917 mg/g(RSD=2.85%,n=3)。结论:本研究优化的醇提工艺稳定可行、提取率较高,适用于独子藤果实中雷公藤红素的提取。
ABSTRACT: OBJECTIVE: To optimize the methanol extraction technology of tripterine in the fruit of Celastrus monospermus. METHODS: HPLC was adopted to determine the content of tripterine. Using extraction time, extraction times, solid-liquid ratio as investigation factors, extraction rate of tripterine as investigation index, orthogonal test was designed, and verification test was conducted. RESULTS: The optimal methanol extraction technology was as follow as 45% methanol extracting 2 h each time with solid-liquid ratio of 1 ∶ 8 for 3 times. In the verification test, the average extraction rate of tripterine was 0.917 mg/g (RSD=2.85%, n=3). CONCLUSIONS: The optimized methanol extraction technology is stable and feasible with high extraction rate, and is suitable for the extraction of tripterine in the fruit of C. monospermus.
期刊: 2017年第28卷第19期
作者: 许启荣,周琼,张紫萍,陈纠
AUTHORS: XU Qirong,ZHOU Qiong,ZHANG Zhiping,CHEN Jiu
关键字: 独子藤;雷公藤红素;单因素;正交试验;高效液相色谱法
KEYWORDS: Celastrus monospermus; Tripterine; Single factor; Orthogonal test; HPLC
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