HPLC法同时测定祛瘀止痛合剂中6种成分的含量
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篇名: HPLC法同时测定祛瘀止痛合剂中6种成分的含量
TITLE:
摘要: 目的:建立同时测定祛瘀止痛合剂中芍药苷、柚皮苷、新橙皮苷、三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量的方法。方法:采用高效液相色谱法。色谱柱为Waters Xbridge C18,流动相为0.1%磷酸-乙腈(梯度洗脱),流速为1.0 mL/min,检测波长为203 nm,柱温为20 ℃,进样量为5 μL。结果:芍药苷、柚皮苷、新橙皮苷、三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1检测进样量线性范围分别为0.273 4~2.734 μg(r=0.999 9)、0.119 1~1.191 μg(r=0.999 9)、0.081 5~0.815 μg(r=0.999 9)、0.622 8~6.228 μg(r=0.999 9)、0.807 2~8.072 μg(r=0.999 9)、1.036 4~10.364 μg(r=0.999 9);定量限分别为0.082 0、0.029 8、0.028 5、0.436 0、0.403 6、0.310 9 μg,检测限分别为0.027 9、0.009 5、0.010 2、0.124 6、0.121 1、0.093 3 μg;精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为97.85%~100.34%(RSD=0.81%,n=6)、98.14%~101.22%(RSD=1.09%,n=6)、98.42%~102.15%(RSD=1.29%,n=6)、97.77%~100.25%(RSD=0.96%,n=6)、97.32%~99.53%(RSD=0.81%,n=6)、98.28%~101.51%(RSD=1.11%,n=6)。结论: 该方法简便快速、稳定可行、重复性好,可用于祛瘀止痛合剂中6种成分含量的同时测定。
ABSTRACT: OBJECTIVE: To establish a method for simultaneous determination of paeoniflorin, naringin, neohesperidin, notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Quyu zhitong mixture. METHODS: HPLC method was adopted. The determination was performed on Waters Xbridge C18 column with mobile phase consisted of 0.1% phosphoric acid-acetonitrile (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 203 nm, and column temperature was 20 ℃. The sample size was 5 μL. RESULTS: The linear ranges of paeoniflorin, naringin, neohesperidin, notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were 0.273 4-2.734 μg(r=0.999 9),0.119 1-1.191 μg(r=0.999 9),0.081 5-0.815 μg(r=0.999 9),0.622 8-6.228 μg(r=0.999 9),0.807 2-8.072 μg(r=0.999 9),1.036 4-10.364 μg(r=0.999 9). The limits of quantitation were 0.082 0, 0.029 8, 0.028 5, 0.436 0, 0.403 6, 0.310 9 μg. The limits of detection were 0.027 9, 0.009 5, 0.010 2, 0.124 6, 0.121 1,0.093 3 μg. RSDs of precision, stability and reproducibility tests were lower than 2.0%. Recoveries were 97.85%-100.34%(RSD=0.81%,n=6),98.14%-101.22%(RSD=1.09%,n=6),98.42%-102.15%(RSD=1.29%,n=6), 97.77%-100.25%(RSD=0.96%,n=6),97.32%-99.53%(RSD=0.81%,n=6) and 98.28%-101.51% (RSD=1.11%,n=6). CONCLUSIONS: The method is simple, rapid, stable, feasible and reproducible, and can be used for the simultaneous determination 6 components in Quyu zhitong mixture.
期刊: 2017年第28卷第18期
作者: 李洁环,王洛临,郭鸣,徐文杰,陈雪婷,李智勇
AUTHORS: LI Jiehuan,WANG Luolin,GUO Ming,XU Wenjie,CHEN Xueting,LI Zhiyong
关键字: 高效液相色谱法;祛瘀止痛合剂;芍药苷;柚皮苷;新橙皮苷;三七皂苷R1;人参皂苷Rg1;人参皂苷Rb1
KEYWORDS: HPLC; Quyu zhitong mixture; Paeoniflorin; Naringin; Neohesperidin; Notoginsenoside R1; Ginsenoside Rg1; Ginsenoside Rb1
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