HPLC法同时测定栀子金花分散片中7种成分的含量
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篇名: | HPLC法同时测定栀子金花分散片中7种成分的含量 |
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摘要: | 目的:建立同时测定栀子金花分散片中栀子苷、黄芩苷、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚含量的方法。方法:采用高效液相色谱法。色谱柱为 Dimonsil C18,流动相为甲醇-0.05%磷酸溶液(梯度洗脱),流速为0.8 mL/min,检测波长为254 nm,柱温为25 ℃,进样量为20 μL。结果:栀子苷、黄芩苷、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚检测进样量线性范围分别为0.032 3~0.323 μg(r=0.999 8)、0.137 4~1.374 μg(r=0.999 9)、0.003 72~0.037 2 μg(r=0.999 7)、0.006 9~0.069 μg(r=0.999 5)、0.003 32~0.033 2 μg(r=0.999 7)、0.008 64~0.086 4 μg(r=0.999 7)、0.001 22~0.012 2 μg(r=0.999 5);定量限分别为0.032 1、0.137 4、0.003 72、0.006 7、0.003 30、0.008 64、0.001 22 μg,检测限分别为0.009 5、0.004 1、0.001 2、0.002 0、0.001 0、0.002 6、0.000 3 μg;精密度、稳定性、重复性试验的RSD<3%;加样回收率分别为96.54%~99.52%(RSD=1.17%,n=6)、97.23%~101.23%(RSD=1.36%,n=6)、97.22%~101.25%(RSD=1.83%,n=6)、97.32%~100.23%(RSD=1.09%,n=6)、97.99%~102.71%(RSD=1.73%,n=6)、96.99%~100.23%(RSD=1.21%,n=6)、96.99%~103.01%(RSD=2.31%,n=6)。结论:该方法操作简便、重复性好,可用于同时测定栀子金花分散片中7种成分的含量。 |
ABSTRACT: | OBJECTIVE: To develop a method for simultaneous determination of geniposide, baicalin, aloe-emodin, rhein, emodin, chrysophanol and physcion in Zhizi jinhua dispersible tablets. METHODS: HPLC method was adopted. The determination was performed on Dimonsil C18 column with mobile phase consisted of methanol-0.05% phosphoric acid (gradient elution) at the flow rate of 0.8 mL/min. The detection wavelength was set at 254 nm, and the column temperature was 25 ℃. The sample size was 20 μL. RESULTS: The linear ranges of geniposide, baicalin, aloe-emodin, rhein, emodin, chrysophanol and physcion were 0.032 3-0.323 μg(r=0.999 8),0.137 4-1.374 μg(r=0.999 9),0.003 72-0.037 2 μg(r=0.999 7), 0.006 9-0.069 μg(r=0.999 5), 0.003 32-0.033 2 μg(r=0.999 7), 0.008 64-0.086 4 μg(r=0.999 7) and 0.001 22-0.012 2 μg(r=0.999 5), respectively. The limits of quantitation were 0.032 1,0.137 4, 0.003 72, 0.006 7, 0.003 30, 0.008 64, 0.001 22 μg, respectively. The limits of detection were 0.009 5, 0.004 1, 0.001 2, 0.002 0, 0.001 0, 0.002 6, 0.000 3 μg, respectively. RSDs of precision, stability and reproducibility tests were all lower than 3%. The average recoveries were 96.54%-99.52%(RSD=1.17%,n=6),97.23%-101.23% (RSD=1.36%,n=6),97.22%-101.25%(RSD=1.83%,n=6),97.32%-100.23%(RSD=1.09%,n=6), 97.99%-102.71%(RSD=1.73%, n=6), 96.99%-100.23%(RSD=1.21%,n=6), 96.99%-103.01%(RSD=2.31%,n=6), respectively. CONCLUSIONS: The methods is simple and reproducible. It can be used for the content determination of 7 components in Zhizi jinhua dispersible tablets. |
期刊: | 2017年第28卷第18期 |
作者: | 肖娅,李晶,常金花,陈威,刘翠哲,刘喜纲 |
AUTHORS: | XIAO Ya,LI Jing,CHANG Jinhua,CHEN Wei,LIU Cuizhe,LIU Xigang |
关键字: | 高效液相色谱法;栀子金花分散片;栀子苷;黄芩苷;芦荟大黄素;大黄酸;大黄素;大黄酚;大黄素甲醚;含量 |
KEYWORDS: | HPLC; Zhizi jinhua dispersible tablets; Geniposide; Baicalin; Aloe-emodin; Rhein; Emodin; Chrysophanol; Physcion; Content |
阅读数: | 283 次 |
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