一测多评法同时测定山银花药材中5种皂苷类成分的含量
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篇名: | 一测多评法同时测定山银花药材中5种皂苷类成分的含量 |
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摘要: | 目的:建立同时测定山银花药材中5种皂苷类成分含量的方法。方法: 采用高效液相色谱法,以灰毡毛忍冬皂苷乙为内标,计算其对灰毡毛忍冬皂苷甲、川续断皂苷乙、灰毡毛忍冬次皂苷甲和灰毡毛忍冬次皂苷乙的相对校正因子(RCF),通过RCF计算上述4种皂苷类成分的含量;以外标法测定的上述皂苷类成分含量作实测值,比较计算值与实测值的差异。结果:灰毡毛忍冬皂苷甲、灰毡毛忍冬皂苷乙、川续断皂苷乙、灰毡毛忍冬次皂苷甲和灰毡毛忍冬次皂苷乙检测进样量线性范围分别为0.316~6.32 μg(r=0.997 3)、0.453~9.06 μg(r=0.998 2)、0.231~4.62 μg(r=0.999 6)、0.342~6.84 μg(r=0.998 4)、0.147~2.94 μg(r=0.996 1);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率试验的RSD 分别为97.74%~104.51%(RSD=2.37%,n=6)、96.70%~103.20%(RSD=2.37%,n=6)、96.12%~103.61%(RSD=2.45%,n=6)、98.80%~104.70%(RSD=2.32%,n=6)、99.21%~102.92%(RSD=1.39%,n=6)。计算值与实测值差异无统计学意义(P>0.05)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于山银花药材中5种皂苷类成分含量的同时测定。 |
ABSTRACT: | OBJECTIVE: To establish the method for simultaneous determination of 5 saponins in Lonicerae Flos. METHODS: Using macranthoidin B as a reference, HPLC method was adopted to calculate the relative correction factor (RCF) of it to macranthoidin A, dipsacoside B, macranthoside A and macranthoside B. The contents of above 4 saponins were calculated through RCF. Using the contents of saponins determined by external standard method as measured value, the calculated value was compared with measured value. RESULTS: The linear ranges of macranthoidin A, macranthoidin B, dipsacoside B, macranthoside A and macranthoside B were 0.316-6.32 μg(r=0.997 3), 0.453-9.06 μg(r=0.998 2),0.231-4.62 μg(r=0.999 6), 0.342-6.84 μg(r=0.998 4)and 0.147-2.94 μg(r=0.996 1), respectively. RSDs of precision, stability and reproducibility tests were all lower than 2.0%. The recoveries were 97.74%-104.51%(RSD=2.37%,n=6)、96.70%-103.20%(RSD=2.37%,n=6)、96.12%-103.61%(RSD=2.45%,n=6)、98.80%-104.70%(RSD=2.32%,n=6)、99.21%-102.92%(RSD=1.39%,n=6), respectively. There was no statistical significance between calculated value and measured value (P>0.05). CONCLUSIONS: The method is simple, precise, stable and reproducible. It can be used for the determination of saponins in Lonicerae Flos. |
期刊: | 2017年第28卷第18期 |
作者: | 孙玲,樊晓兰,郭绮,张小蒙,陈磊 |
AUTHORS: | SUN Ling,FAN Xiaolan,GUO Qi,ZHANG Xiaomeng,CHEN Lei |
关键字: | 一测多评法;山银花;外标法;相对校正因子;灰毡毛忍冬皂苷乙 |
KEYWORDS: | QAMS; Lonicerae Flos; External standard method; Relative correction factor; Macranthoidin B |
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