星点设计-响应面法优化山楂有机酸的提取工艺
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篇名: 星点设计-响应面法优化山楂有机酸的提取工艺
TITLE:
摘要: 目的:优化山楂有机酸的提取工艺。方法:在单因素试验基础上,以液料比、提取时间及乙醇体积分数为自变量,以有机酸得率为因变量,采用星点设计-响应面法优化山楂有机酸提取工艺。结果:最优提取工艺条件为山楂按液料比18.5 ∶ 1加入75%乙醇,回流提取2次,每次2.0 h;验证试验的有机酸得率平均值为5.22%(RSD=2.70%,n=3),与模型预测值(5.13%)的相对误差为1.75%。 结论:优化建立的山楂有机酸提取工艺方法简单,重现性和可预测性较好。
ABSTRACT: OBJECTIVE: To optimize the extraction technology for organic acids in Crataegi fructus. METHODS: Based on single factor test, using liquid-solid ratio, extraction time and ethanol volume fraction as independent variables, extraction yield of organic acids as dependent variable, central composite design-response surface method was used to optimize the extraction technology of organic acids in Crataegi fructus. RESULTS: The optimal extraction technology was as follow as liquid-solid ratio of 18.5 ∶ 1, adding 75% ethanol, reflux extraction twice, 2.0 h each time. Average extraction yield of organic acids in verification test was 5.22% (RSD=2.70%, n=3), with 1.75% relative error of the predicted value (5.13%). CONCLUSIONS: Optimized extraction technology for organic acids in Crataegi fructus is simple, with good reproducibility and predictability.
期刊: 2017年第28卷第16期
作者: 熊科元,万丹娜,邵峰,赵海平,刘荣华,黄慧莲,杨明
AUTHORS: XIONG Keyuan,WAN Danna,SHAO Feng,ZHAO Haiping,LIU Ronghua,HUANG Huilian,YANG Ming
关键字: 山楂;有机酸;提取工艺;星点设计-响应面法
KEYWORDS: Crataegi fructus; Organic acids; Extraction technology; Central composite design-response surface method
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