丛枝菌根真菌对云木香根产量及其木香烃内酯和去氢木香烃内酯含量的影响
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篇名: 丛枝菌根真菌对云木香根产量及其木香烃内酯和去氢木香烃内酯含量的影响
TITLE:
摘要: 目的:研究丛枝菌根(AM)真菌在人工栽培条件下对云木香根产量及其有效成分含量的影响。方法:采用室温盆栽方法,设不种接AM真菌组为CK组,设接种阿密蔻菌根组为AM组,每组5个重复,每个重复1盆,共5盆,每盆栽种云木香3株。采用文献方法计算菌根侵染率和侵染强度,采用称重法测定云木香根产量,采用高效液相色谱法测定木香烃内酯、去氢木香烃内酯的含量。结果:AM组与CK组的侵染率分别为(86.22±3.28)%、0(P<0.05),侵染强度分别为(52.12±2.14)%、0(P<0.05);两组间根增质量率和木香烃内酯、去氢木香烃内酯及总内酯的含量比较差异有统计学意义(P<0.05)。与CK组比较,AM组云木香根产量提高了(50.96±2.12)%,云木香根中木香烃内酯、去氢木香烃内酯及总内酯的含量分别增加了65.55%、97.42%、85.42%,内酯类成分间的结构比有所改变。结论:外源性AM真菌的加入能明显提高云木香根的品质。
ABSTRACT: OBJECTIVE: To study the effects of Arbuscular mycorrhizal (AM) fungi on yield and its active ingredients contents of Saussurea costus in artificial cultivation. METHODS: Through the pot method at room temperature, not inoculated AM fungi group was regarded as CK group, inoculated AMykor mycorrhiza group as AM group, 5 repeated in each group, one pot in each repeated, there were 5 pots in all, 3 strains of S. costus in each pot. Literature establishment method was used to calculate the mycorrhizal colonization rate and colonization intensity. Weighing method was adopted to determine the yield and HPLC was conducted to determine the content of costunolide and dehydrocostuslactone. RESULTS: Colonization rates of AM group and CK group were (86.22±3.28)%, 0 (P<0.05); colonization intensities were (52.12±2.14)%, 0 (P<0.05). There were statistical significances in weighing rate of root, contents of costunolide, dehydrocostuslactone and total lactones in 2 groups (P<0.05). Compared with CK group, the yield increase in AM group (50.96±2.12)%, contents of costunolide, dehydrocostus lactone and total lactones increased 65.55%, 97.42%, 85.42%, respectively. And there was a certain change on the structure ratio of lactones. CONCLUSIONS: Inoculating exogenous AM fungi can significantly improve the root quality of S. costus.
期刊: 2017年第28卷第16期
作者: 张杰,潘兴娇,罗静,周浓,谢娥,杨敏
AUTHORS: ZHANG Jie,PAN Xingjiao,LUO Jing,ZHOU Nong,XIE E,YANG Min
关键字: 云木香;丛枝菌根真菌;产量;木香烃内酯;去氢木香烃内酯
KEYWORDS: Saussurea costus; Arbuscular mycorrhizal fungi; Yield; Costunolide; Dehydrocostuslactone
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