超声波-酶法提取积雪草中积雪草总苷的工艺研究
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篇名: 超声波-酶法提取积雪草中积雪草总苷的工艺研究
TITLE:
摘要: 目的:优化积雪草中积雪草总苷的提取工艺。方法:以积雪草苷和羟基积雪草苷含量之和为考察指标,采用单因素试验和正交试验对酶用量、酶解时间、酶解温度、乙醇体积分数、液料比、超声提取时间进行考察,优化超声波-酶法提取积雪草中积雪草总苷的工艺条件并进行验证试验。结果:最优提取工艺为纤维素酶用量12 mg/g,以10倍液料比加入60%乙醇,60 ℃酶解60 min,超声提取50 min;验证试验中积雪草总苷平均提取率为1.92%(RSD=1.83%,n=3)。结论:超声波-酶法用于积雪草中积雪草总苷的提取,工艺稳定、可行。
ABSTRACT: OBJECTIVE: To optimize the extraction technology of asiaticosides from Centella asiatica. METHODS: Using total amounts of asiaticoside and hydroxy asiaticoside as investigation indexes, single factor and orthogonal test were used to investigate the enzyme amount, enzymolysis time, enzymolysis temperature, ethanol volume fraction, liquid material ratio and ultrasonic extraction time and optimize the extraction technology of asiaticosides from C. asiatica by ultrasonic-enzyme method, and verification test was conducted. RESULTS: Optimal extraction technology was as follow as cellulose dosage of 12 mg/g, 10-fold liquid material ratio added into 60% ethanol, enzyme hydrolysis for 60 min at 60 ℃, ultrasonic assisted extraction for 50 min. Average extraction rate of total asiaticosides was 1.92% (RSD=1.83%, n=3) in verification test. CONCLUSIONS: Ultrasonic-enzyme method is stable and feasible for the extraction technology of asiaticosides from C. asiatica.
期刊: 2017年第28卷第13期
作者: 张丹丹,聂绪强,张涵,赵久凤,石秀君,杨建文
AUTHORS: ZHANG Dandan,NIE Xuqiang,ZHANG Han,ZHAO Jiufeng,SHI Xiujun,YANG Jianwen
关键字: 积雪草;积雪草苷;羟基积雪草苷;超声波;纤维素酶;提取工艺;正交试验
KEYWORDS: Centella asiatica; Asiaticoside; Hydroxy asiaticoside; Ultrasonic; Cellulose; Extraction technology; Orthogonal test
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