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半仿生酶法提取柿叶中总黄酮的工艺筛选及优化
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| 篇名： | 半仿生酶法提取柿叶中总黄酮的工艺筛选及优化 |
| TITLE： | |
| 摘要： | 目的：筛选提取柿叶中黄酮的方法及最优提取工艺。方法：以浸膏质量、黄酮含量为指标，比较乙醇回流法、酶法（纤维素酶、β-葡聚糖酶及二者等量混合组成的复合酶）、半仿生法和半仿生酶法（同前3种酶）提取柿叶中总黄酮的效果。以黄酮含量为指标，以料液比、回流温度和回流时间为因素，设计正交试验优化半仿生酶法提取柿叶中黄酮的工艺条件，并进行验证试验。结果：在4种提取方法中，以半仿生酶法得到的浸膏质量和黄酮含量最高，并以复合酶效果最好；优化的半仿生酶法提取工艺条件为料液比1 ∶ 14、回流温度50 ℃、回流时间2.0 h；验证试验中3次黄酮提取率分别为5.9％、5.8％、5.9％（RSD＝0.98％，n＝3）。结论：优化后的半仿生酶法提取柿叶中黄酮，提取率高且工艺稳定。 |
| ABSTRACT： | OBJECTIVE： To screen the method for extracting total flavonoids in persimmon leaves and optimize extraction technology. METHODS： Using extract quality and flavonoids content as indexes， the effects of extracting total flavonoids in persimmon leaves by ethanol refluxing method， enzyme method （cellulase， β-glucanase and complex enzyme mixed by equal amounts of both）， semi-bionic method and semi-bionic-enzyme method （the same enzymes） were compared. Using flavonoids content as index， solid-liquid radio， reflux temperature， reflux time as factors， orthogonal test was designed to optimize the extraction technology conditions of flavonoids in persimmon leaves by semi-bionic-enzyme method， and the verification test was conducted. RESULTS： The extract quantity and flavonoids content by semi-bionic-enzyme method was the highest among the 4 extraction methods， and the complex enzyme was the most suitable； the optimized extracting condition of semi-bionic-enzyme method were as follows as solid-liquid radio of 1 ∶ 14， reflux temperature of 50 ℃， reflux time of 2.0 h； extraction rates of flavonoids in 3 verification tests were 5.9％， 5.8％， 5.9％ （RSD＝0.98％， n＝3）. CONCLUSIONS： The optimized semi-bionic-enzyme method is efficient and stable in extracting flavonoids in persimmon leaves. |
| 期刊： | 2017年第28卷第13期 |
| 作者： | 薛璇玑，罗俊，张新新，钱春香，赵晶，郭增军 |
| AUTHORS： | XUE Xuanji，LUO Jun，ZHANG Xinxin，QIAN Chunxiang，ZHAO Jing，GUO Zengjun |
| 关键字： | 半仿生酶法；柿叶；黄酮；提取；正交试验；工艺优化 |
| KEYWORDS： | Semi-bionic-enzyme method； Persimmon leaves； Favonoids； Extraction； Orthogonal test； Technology optimization |
| 阅读数： | 334 次 |
| 本月下载数： | 7 次 |
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