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淫羊藿苷对自发性高血压大鼠肾损伤的改善作用及机制研究
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| 篇名： | 淫羊藿苷对自发性高血压大鼠肾损伤的改善作用及机制研究 |
| TITLE： | |
| 摘要： | 目的：观察淫羊藿苷（ICA）对自发性高血压大鼠（SHR）肾组织病理损伤的影响，并基于核因子κB（NF-κB）信号通路研究其作用机制。方法：将21只SHR随机均分为模型组和ICA低、高剂量组（20、40 mg/kg，记为 ICA-L、ICA-H组），另取7只同源京都大鼠（WKY）为对照组。各组大鼠ig给药，每日2次，连用11周；对照组和模型组大鼠ig等体积双蒸水。观察各组大鼠肾组织病理学变化；蛋白质印迹法检测肾组织磷酸化NF-κB-p65（p-NF-κB-p65）、NF-κB抑制蛋白（IκB）、肿瘤坏死因子α （TNF-α）蛋白的表达。结果：与对照组比较，模型组大鼠肾结构出现紊乱，肾小球囊腔狭窄且不规则；IκB蛋白的表达明显下调，p-NF-κB-p65、TNF-α蛋白的表达均明显上调（P＜0.01）。与模型组比较，ICA-L、ICA-H组大鼠肾组织上述病理变化有所改善；ICA-L、ICA-H组IκB蛋白的表达均明显上调（P＜0.05或P＜0.01），ICA-H组大鼠p-NF-κB-p65、TNF-α蛋白的表达均明显下调（P＜0.01），ICA-L组大鼠p-NF-κB-p65蛋白表达明显下调（P＜0.05），而ICA-L组大鼠TNF-α蛋白表达差异无统计学意义（P＞0.05）。结论： ICA具有改善SHR肾病理性损伤的作用，其机制可能与抑制NF-κB信号通路相关。 |
| ABSTRACT： | OBJECTIVE： To observe the effect of pathological lesion of renal tissue in rats with spontaneous hypertension （SHR）， and study its mechanisms based on nuclear factor κB （NF-κB） signaling pathway. METHODS： 21 SHR were randomly divided into model group and ICA low-dose， high-dose groups （20， 40 mg/kg， denoted by ICA-L， ICA-H groups）； other 7 homologous Kyoto rats （WKY） were regarded as control group. All rats were intragastrically administrated， twice a day， for 11 weeks， rats in control group and model group received equal volume of double distilled water， ig. Pathological changes in renal tissue in each group were observed； Western blot method was used to detect protein expressions of p-NF-κB-p65， IκB and TNF-α in renal tissue. RESULTS： Compared with control group， model group showed disorder renal structure， narrow and irregular glomerular cysts； the protein expression of IκB was significantly down-regulated， protein expressions of p-NF-κB-p65 and TNF-α were significantly up-regulated （P＜0.01）. Compared with model group， the above-mentioned changes of rats showed improvement in ICA-L， ICA-H groups； the protein expression of IκB was significantly up-regulated in ICA-L， ICA-H groups （P＜0.05 or P＜0.01）； the protein expressions of p-NF-κB-p65 and TNF-α were significantly down-regulated （P＜0.01） in ICA-H groups； p-NF-κB-p65 protein expression was significantly down-regulated in ICA-L group （P＜0.05）； while there was no significant difference in TNF-α protein expression in ICA-L group （P＞0.05）. CONCLUSIONS： ICA plays a role in improving renal pathological lesion in SHR， and the mechanism may be related to inhibiting NF-κB signaling pathway. |
| 期刊： | 2017年第28卷第10期 |
| 作者： | 朱玲，钱志强，李叶丽，王俊逸，杨华，杨丹莉 |
| AUTHORS： | ZHU Ling，QIAN Zhiqiang，LI Yeli，WANG Junyi，YANG Hua，YANG Danli |
| 关键字： | 淫羊藿苷；自发性高血压大鼠；肾组织；磷酸化核因子κB-p65；肿瘤坏死因子α |
| KEYWORDS： | Icariin； Spontaneously hypertension rats； Renal tissue； p-NF-κB-p65； TNF-α |
| 阅读数： | 361 次 |
| 本月下载数： | 2 次 |
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