艾纳香及其伪品假东风草的HPLC指纹图谱研究
x

请在关注微信后,向客服人员索取文件

篇名: 艾纳香及其伪品假东风草的HPLC指纹图谱研究
TITLE:
摘要: 目的:建立艾纳香及其伪品假东风草的高效液相色谱(HPLC)指纹图谱。方法:采用HPLC法。色谱柱为Uitimate-C18,流动相为乙腈-0.05%磷酸溶液(梯度洗脱),流速为0.6 mL/min,检测波长为270 nm,柱温为25 ℃,进样量为7 μL。以槲皮素为参照物,对16批艾纳香药材和5批假东风草药材采用《中药色谱指纹图谱相似度评价系统》(2004 A版)进行共有峰指认和相似度分析。结果:16批艾纳香药材有61个共有峰,相似度为0.931~0.995,明显高于5批假东风草药材的相似度。结论:该研究所建指纹图谱可为艾纳香的鉴别和质量评价提供参考。
ABSTRACT: OBJECTIVE: To establish the HPLC fingerprint for Blumea balsamifera and its fake B. riparia. METHODS: HPLC was performed on the column of Uitimate-C18 with mobile phase of acetonitrile-0.05% phosphoric acid (gradient elution) at a flow rate of 0.6 mL/min, detection wavelength was 270 nm, column temperature was 25 ℃, and injection volume was 7 μL. Using quercetin as a reference, Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine (2004 A edition)was used for the common peaks identification and similarity analysis of 16 batches of B. balsamifera and 5 batches of B. riparia. RESULTS: There were 61 common peaks in the 16 batches of B. balsamifera, similarity degree was 0.931-0.995, which was higher than the similarity degree of 5 batches of B. riparia. CONCLUSIONS: The established fingerprint can provide reference for the identification and quality evaluation of B. balsamifera.
期刊: 2017年第28卷第9期
作者: 冯华,杨烨,王祥培,吴红梅,杨贤友
AUTHORS: FENG Hua,YANG Ye,WANG Xiangpei,WU Hongmei,YANG Xianyou
关键字: 艾纳香;假东风草;高效液相色谱法;指纹图谱;鉴别
KEYWORDS: Blumea balsamifera; Blumea riparia; HPLC; Fingerprint; Identification
阅读数: 289 次
本月下载数: 4 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!