HPLC法同时测定茵胆平肝胶囊中6种成分的含量
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篇名: HPLC法同时测定茵胆平肝胶囊中6种成分的含量
TITLE:
摘要: 目的:建立同时测定茵胆平肝胶囊中绿原酸、栀子苷、龙胆苦苷、阿魏酸、黄芩苷、甘草酸铵含量的方法。方法:采用高效液相色谱法。色谱柱为Wonda SilTM-C18,流动相为乙腈-0.4%磷酸溶液(梯度洗脱),流速为0.8 mL/min,检测波长分别为325 nm(绿原酸和阿魏酸)、250 nm(栀子苷和甘草酸铵)、275 nm(龙胆苦苷和黄芩苷),柱温为30 ℃。结果:绿原酸、栀子苷、龙胆苦苷、阿魏酸、黄芩苷、甘草酸铵检测进样量线性范围分别为0.087~3.480 μg(r=0.999 8)、0.201~8.040 μg(r=0.999 7)、0.200~8.000 μg(r=0.999 5)、0.016~0.640 μg(r=0.999 9)、0.105~4.200 μg(r=0.999 9)、0.028~1.120 μg(r=0.999 5);定量限分别为1.31、0.75、1.14、1.25、0.94、0.98 ng,检测限分别为0.87、0.67、0.96、0.93、0.60、0.88 ng;精密度、稳定性、重复性试验的RSD<2.0 %;加样回收率分别为99.9%~101.9%(RSD=0.7%,n=6)、98.7%~100.9%(RSD=0.9%,n=6)、98.1%~101.5%(RSD=1.4%,n=6)、98.5%~101.3%(RSD=1.3%,n=6)、98.5%~101.7%(RSD=1.2%,n=6)、98.2%~101.4%(RSD=1.2%,n=6)。结论:该方法简便、结果准确,适用于茵胆平肝胶囊中绿原酸、栀子苷、龙胆苦苷、阿魏酸、黄芩苷、甘草酸铵含量的同时测定。
ABSTRACT: OBJECTIVE: To develop a method for simultaneous determination of chlorogenic acid, geniposide, gentiopicroside, ferulic acid, baicalin and ammonium glycyrrhizinate in Yindan pinggan capsule. METHODS: HPLC method was adopted. The separation was performed on Wonda SilTM-C18 column with mobile phase consisting of acetonitrile-0.4% phosphoric acid solution (gradient elution) at the flow rate of 0.8 mL/min. The detection wavelength were set at 325 nm (chlorogenic acid, ferulic acid), 250 nm (geniposide, ammonium glycyrrhizinate) and 275 nm (gentiopicroside, baicalin). The column temperature was  30 ℃. RESULTS: The linear ranges were 0.087-3.480 μg for chlorogenic acid (r=0.999 8), 0.201-8.040 μg  for geniposide (r=0.999 7), 0.200-8.000 μg  for gentiopicroside (r=0.999 5), 0.016-0.640 μg for ferulic acid (r=0.999 9), 0.105-4.200 μg for baicalin (r=0.999 9) and 0.028-1.120 μg for ammonium glycyrrhizinate (r=0.999 5), respectively. The limits of quantitation were 1.31, 0.75, 1.14, 1.25, 0.94, 0.98 ng, and the limits of detection were 0.87, 0.67, 0.96, 0.93, 0.60, 0.88 ng, respectively. RSDs of precision, stability and reproducibility tests were lower than 2.0%; recoveries were 99.9%-101.9% (RSD=0.7%,n=6), 98.7%-100.9%(RSD=0.9%,n=6), 98.1%-101.5% (RSD=1.4%,n=6), 98.5%-101.3%(RSD=1.3%,n=6), 98.5%-101.7% (RSD=1.2%,n=6), 98.2%-101.4%(RSD=1.2%,n=6), respectively. CONCLUSIONS: The method is simple and accurate ,can be used for simultaneous determination of chlorogenic acid, geniposide, gentiopicroside, ferulic acid, baicalin and ammonium glycyrrhizinate in Yindan pinggan capsule.
期刊: 2017年第28卷第9期
作者: 张宏梅,昌盛,崔佰吉,李星梅,冯宪敏
AUTHORS: ZHANG Hongmei,CHANG Sheng,CUI Baiji,LI Xingmei,FENG Xianmin
关键字: 茵胆平肝胶囊;绿原酸;栀子苷;龙胆苦苷;阿魏酸;黄芩苷;甘草酸铵;高效液相色谱法;含量测定
KEYWORDS: Yindan pinggan capsule; Chlorogenic acid; Geniposide; Gentiopicroside; Ferulic acid; Baicalin; Ammonium glycyrrhizinate; HPLC; Content determination
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