高效阳离子交换色谱法测定尿素[13C]胶囊中尿素的含量
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篇名: 高效阳离子交换色谱法测定尿素[13C]胶囊中尿素的含量
TITLE:
摘要: 目的:采用高效阳离子交换色谱法测定尿素[13C]胶囊中尿素的含量。方法:色谱柱为 Zorbax 300 SCX,流动相为乙腈-0.1%磷酸溶液(20 ∶ 80,V/V),流速为1.0 mL/min,检测波长为200 nm,柱温为35 ℃,进样量为 20 μL。结果:尿素检测质量浓度线性范围为0.003 9~1.003 0 mg/mL(r=0.999 7);定量限为 3.918 μg/mL,检测限为 0.975 μg/mL;精密度、稳定性、重复性试验的RSD<2.0%;加样回收率为99.3%~101.0%(RSD=0.67%,n=9)。结论:该方法简便、快速、灵敏,适用于尿素[13C]胶囊中尿素的含量测定。
ABSTRACT: OBJECTIVE: To determine the content of urea in Urea [13C] capsules by high performance cation-exchange chromatography (HPCEC). METHODS: The determination was performed on Zorbax 300 SCX column with mobile phases consisting of acetonitrile-0.1% phosphoric acid (20 ∶ 80,V/V) at the flow rate of 1.0 mL/min. The detection wavelength was set at 200 nm and column temperature was 35 ℃. The sample size was 20 μL. RESULTS: The linear range of urea was 0.003 9-1.003 0 mg/ml(r=0.999 7). The limit of quantitation was 3.918 μg/mL and the limit of detection was 0.975 μg/mL. RSDs of precision, stability and repetitive test were all lower than 2.0%. The recovery ranged 99.3%-101.0% (RSD=0.67%,n=9). CONCLUSIONS: The method is simple, rapid, sensitive and suitable for the content determination of urea in Urea [13C] capsules.
期刊: 2017年第28卷第9期
作者: 刘桂霞,姚静,左利民,李盼盼,山广志,姜威
AUTHORS: LIU Guixia,YAO Jing,ZUO Limin,LI Panpan,SHAN Guangzhi,JIANG Wei
关键字: 尿素[13C]胶囊;尿素;含量测定;阳离子交换法
KEYWORDS: Urea [13C] capsules; Urea; Content determination; High performance cation-exchange chromatography
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