LC-MS/MS法和EMIT法测定人血清中丙戊酸浓度的对比研究-中国药房

LC-MS/MS法和EMIT法测定人血清中丙戊酸浓度的对比研究

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篇名：	LC-MS/MS法和EMIT法测定人血清中丙戊酸浓度的对比研究
TITLE：
摘要：	目的：比较液相色谱-串联质谱（LC-MS/MS）法与酶放大免疫（EMIT）法测定人血清中丙戊酸（VPA）浓度的差异，为临床治疗药物监测提供参考。方法：分别采用LC-MS/MS法和EMIT法对144份住院或门诊患者血清样品中的VPA浓度进行检测，采用配对t检验、Pearson相关性分析和Bland-Altman偏差图等方法对其检测结果的差异进行评价。结果：LC-MS/MS法和EMIT法的检测结果呈正相关（r＝0.924，P＜0.05）；两者的回归方程为cEMIT＝0.920 7cLC-MS/MS－1.114 4（r＝0.924）；LC-MS/MS法和EMIT法测得的VPA平均血药浓度分别为（49.9±21.2）、（54.9±21.3）μg/mL，组间比较差异有统计学意义（P＜0.05）；EMIT法检测结果比LC-MS/MS法偏高8.3 μg/mL，95％置信区间为（－13.6，18.7）。结论：LC-MS/MS法和EMIT法测定人血清中VPA的浓度相关性良好，但检测结果有一定的差异，建议同一患者长期监测应选用同一种方法。
ABSTRACT：	OBJECTIVE： To compare the difference in the concentration determination of valproic acid （VPA） in human serum by LC-MS/MS and EMIT. METHODS： Both LC-MS/MS and EMIT methods were applied to determine the serum concentration of VPA in 144 inpatients or outpatients. The paired t-test， Pearson correlation analysis， Bland-Altman deviation chart and other methods were used to evaluate the difference in the results of concentration determination. RESULTS： The results of LC-MS/MS method was positively correlated with that of EMIT method （r＝0.924，P＜0.05）； the regression equation of them was cEMIT＝0.920 7cLC-MS/MS-1.114 4（r＝0.924）. Average serum concentrations of VPA determined by LC-MS/MS and EMIT were （49.9±21.2） and （54.9±21.3） μg/mL， with statistical significance （P＜0.05）. The serum concentration of VPA determined by EMIT was higher than that by LC-MS/MS 8.3 μg/mL， 95％ confidence interval was （－13.6，18.7）. CONCLUSIONS： The serum concentration of VPA determined by LC-MS/MS and EMIT have high correlation. But the determination results still have certain difference， it is suggested to use same method for long- term monitering.
期刊：	2017年第28卷第8期
作者：	时海浪，王海东，宋兴发
AUTHORS：	SHI Hailang，WANG Haidong，SONG Xingfa
关键字：	丙戊酸；血药浓度；液相色谱-串联质谱法；酶放大免疫法；差异
KEYWORDS：	Valproic acid； Serum concentration； LC-MS/MS； EMIT； Difference
阅读数：	450 次
本月下载数：	18 次

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