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外源性脱落酸对玄参幼苗耐盐碱能力的影响研究
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| 篇名： | 外源性脱落酸对玄参幼苗耐盐碱能力的影响研究 |
| TITLE： | |
| 摘要： | 目的：研究外源性脱落酸（ABA）对玄参幼苗耐盐碱能力的影响，为在盐碱地种植玄参提供理论依据。方法：50株玄参幼苗分为对照组、盐碱组（75 mmol/L）和盐碱（75 mmol/L）+ ABA低、中、高浓度组（10、50、100 μmol /L），每组10株。移栽20 d起，每4 d干预1次，2周后测定各组植株生长指标（茎高、鲜质量、干质量、存活率）、生理指标[叶绿素、可溶性糖（SS）、可溶性蛋白（SP）和游离脯氨酸（Pro）含量]、丙二醛（MDA）和过氧化氢（H2O2）含量、抗氧化酶活性[超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）活性]、茎和根中Na+和K+含量。结果：与对照组比较，盐碱组玄参幼苗茎高、存活率、SP、茎中Na+和K+、根中Na+含量和CAT、GR活性显著降低，叶绿素、Pro、MDA、H2O2、根中K+含量和SOD、POD活性显著升高（P＜0.05或P＜0.01）。与盐碱组比较，盐碱+ABA中浓度组玄参幼苗茎高、鲜质量、叶绿素含量显著增加，MDA、茎中Na+含量显著降低；低、中浓度组干质量、SS、SP含量和SOD、CAT活性，高浓度组SS含量显著增加；高浓度组茎高、干质量显著降低；低、高浓度组Pro含量显著降低；低、中、高浓度组H2O2、根中Na+含量显著降低，GR活性、茎和根中K+含量显著升高；中、高浓度组POD活性显著降低。结论：施加一定浓度的外源性ABA可有效提高玄参幼苗耐盐碱的能力，增强植株对盐碱环境的适应能力。 |
| ABSTRACT： | OBJECTIVE： To study the effects of exogenous abscisic acid （ABA） on salt-alkali tolerance of Scrophularia ningpoensis seedlings， and to provide theoretic evidence for cultivating S. ningpoensis in salt-alkali soil. METHODS： 50 S. ningpoensis seedlings were divided into control group， salt-alkali group （75 mmol/L）， salt-alkali （75 mmol/L）+ABA low-concentration， medium-concentration and high-concentration groups （10， 50， 100 μmol /L）， with 10 plants in each group. 20 days after transplanting， the plants were sprayed with drugs every 4 days. Growth indexes of the plants （length of stem， fresh weight， dry weight， survival rate）， physiological indexes [the contents of chlorophyll， soluble sugar （SS）， soluble protein （SP） and free proline （Pro）]， the contents of MDA and H2O2， the activities of antioxidase [the activities of SOD， POD， CAT， GR] and the contents of Na+ and K+ in stem and root were determined in each group 2 weeks later. RESULTS： Compared with control group， stem height and survival rate of S. ningpoensis seedlings， SP， the contents of Na+ and K+ in the stem， the contents of Na+， CAT and GR in the roots were all decreased significantly in salt-alkali group； while chlorophyll， Pro， MDA and H2O2 contents， the content of K+ in the roots and the activities of SOD and POD were all increased significantly （P＜0.05 or P＜0.01）. Compared with salt-alkali group， stem height of S. ningpoensis seedlings， fresh weight and chlorophyll content were increased significantly in salt-alkali+ABA medium-concentration group， while MDA and the content of Na+ in the stem were decreased significantly； dry weight， the contents of SS and SP， the activities of SOD and CAT were increased significantly in salt-alkali+ABA low-concentration and medium-concentration groups， while the SS content was increased in salt-alkali+ABA high-concentration group； stem height and dry weight were decreased significantly in salt-alkali+ABA high-concentration group； Pro content of salt-alkali+ABA low-concentration and high-concentration groups were decreased significantly； H2O2， Na+ content in the roots were decreased significantly in salt-alkali+ABA low-concentration， medium-concentration and high-concentration groups； while GR activity， K+ content in the stem and roots were increased significantly； POD activity were decreased significantly in salt-alkali+ABA medium-concentration and high-concentration groups. CONCLUSIONS： The addition of a certain concentration of exogenous ABA can effectively increase salt-alkali tolerance of S. ningpoensis seedlings and strengthen the ability of the plant adapting to salt-alkali environment. |
| 期刊： | 2017年第28卷第4期 |
| 作者： | 王建安，付英杰，王荣华，李艳芝，王欣 |
| AUTHORS： | WANG Jian’an，FU Yingjie，WANG Ronghua，LI Yanzhi，WANG Xin |
| 关键字： | 玄参；盐碱胁迫；外源性脱落酸；生长指标；抗氧化酶 |
| KEYWORDS： | Scrophularia ningpoensis； Salt-alkali stress； Exogenous abscisic acid； Growth indexes； Antioxidas |
| 阅读数： | 387 次 |
| 本月下载数： | 4 次 |
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