补肾活血化痰汤对多囊卵巢综合征模型大鼠卵巢血供及形态的影响-中国药房

补肾活血化痰汤对多囊卵巢综合征模型大鼠卵巢血供及形态的影响

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篇名：	补肾活血化痰汤对多囊卵巢综合征模型大鼠卵巢血供及形态的影响
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摘要：	目的：探讨补肾活血化痰汤对多囊卵巢综合征（PCOS）模型大鼠卵巢血供及形态的影响。方法：取SD大鼠（♀）50只随机分为正常对照组、模型组、阳性对照组[罗格列酮，3 mg/（kg·d）]和补肾活血化痰汤低、高剂量组[5.0、7.5 g/（kg·d）]，每组10只。除正常对照组外，其余各组大鼠均采用Poresky法复制PCOS模型。成模后，各给药组大鼠ig相应药物，正常对照组和模型组大鼠ig生理盐水[10 mL/（kg·d）]，每天1次，连续22 d。给药结束后处死大鼠，记录微血管密度（MVD），检测大鼠卵巢组织血管内皮生长因子（VEGF）、血管紧张素Ⅱ（Ang Ⅱ）1型受体（AT1）和2型受体（AT2）以及G蛋白偶联受体MAS mRNA的表达，并观察卵巢形态变化。结果：与正常对照组比较，模型组和各给药组大鼠MVD均升高，AT1、AT2、MAS mRNA表达均下调（P＜0.05）；模型组大鼠卵巢组织可见囊性窦卵泡、颗粒细胞层减少，卵母细胞消失，黄体组织数量减少等明显病理变化。与模型组比较，各给药组上述指标均得到显著改善，且补肾活血化痰汤低、高剂量组作用均强于阳性对照组（P＜0.05）；各给药组大鼠的卵巢组织病理变化均得到一定改善。结论：补肾活血化痰汤可增加PCOS模型大鼠卵巢组织血供，促进血管生成，促排卵，有助于改善卵巢形态。
ABSTRACT：	OBJECTIVE： To explore the effects of Bushen huoxue huatan decoction on ovarian blood supply and form in model rats with polycystic ovarian syndrome （PCOS）. METHODS： 50 SD female rats were randomized into normal control group， model group， positive control group [rosiglitazone， 3 mg/（kg·d）]， Bushen huoxue huatan decoction low-dose and high-dose groups [5.0， 7.5 g/（kg·d）]， with 10 rats in each group. Except for normal control group， PCOS model was induced in the other groups by Poresky method. After modeling， treatment groups were given relevant medicine intragastrically. The normal control group and model group were given normal saline [10 mL/（kg·d）] intragastrically， once a day， for 22 days. After the end of administration， the rats were sacrificed. The microvessel density （MVD） was recorded. mRNA expression of vascular endothelial growth factor （VEGF）， ovarian angiotensin Ⅱ receptor （Ang Ⅱ） type 1 （AT1） and type 2 （AT2）， G-protein coupled receptor MAS were all detected in ovarian tissue of rats. The ovarian form was observed. RESULTS： Compared with normal control group， MVD of model group and treatment groups were all increased， while mRNA expression of AT1， AT2 and MAS were down-regulated （P＜0.05）； cystic sinus sollicle， the decrease of granular cell layer， egg mother cells disappearance， the decrease of luteal tissue and other obvious pathological changes could be found in ovarian tissue of rats in model group. Compared with model group， above indexes of treatment groups were improved significantly， and the effects of Bushen huoxue huatan decoction low-dose and high-dose groups were better than that of positive control group （P＜0.05）. The pathological changes of ovarian tissue in treatment groups were all improved to certain extent. CONCLUSIONS： Bushen huoxue huatan decoction can improve ovarian blood supply， angiogenesis and ovulation in PCOS rats. It is beneficial to improve ovarian form.
期刊：	2017年第28卷第4期
作者：	王瑞杰，李晖，邱方，王自闯
AUTHORS：	WANG Ruijie，LI Hui，QIU Fang，WANG Zichuang
关键字：	补肾活血化痰汤；多囊卵巢综合征；卵巢形态；卵巢血供；大鼠
KEYWORDS：	Bushen?huoxue?huatan?decoction； Polycystic ovarian syndrome； Ovarian form； Ovarian blood supply； Rats
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