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人参皂苷Rg1配伍乌头碱对体外培养心衰模型心肌细胞的保护作用
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| 篇名： | 人参皂苷Rg1配伍乌头碱对体外培养心衰模型心肌细胞的保护作用 |
| TITLE： | |
| 摘要： | 目的：考察人参皂苷Rg1配伍乌头碱对体外培养心衰模型心肌细胞的保护作用。方法：将新生大鼠心肌细胞分为正常对照组、模型组、阳性对照组（去乙酰毛花苷注射液，1×10－7 mol/L）、人参皂苷Rg1组（1×10－8 mol/L）、乌头碱组（1×10－9 mol/L）以及人参皂苷Rg1与乌头碱不同配比的配伍组（1 ∶ 1、2 ∶ 1、1 ∶ 2，V/V）。除正常对照组外，其余各组细胞均以0.8％戊巴比妥钠诱导心肌细胞心衰模型。成模后，各组细胞给予相应药物培养1 h，然后检测细胞总三磷酸腺苷（ATP）酶、Ca2+-Mg2+-ATP酶和Na+-K+-ATP酶活力；检测细胞培养液中酸性磷酸酶（ACP）、乳酸脱氢酶（LDH）活力和脑钠肽（BNP）、肿瘤坏死因子α（TNF-α）含量以及细胞中总糖原含量。结果：与正常对照组比较，模型组细胞总ATP酶和Ca2+-Mg2+-ATP酶活力明显降低，Na+-K+-ATP酶活力明显升高，培养液中ACP、LDH活力及BNP含量均明显升高（P＜0.05）。与模型组比较，各给药组细胞总ATP酶活力显著升高，细胞培养液中LDH活力显著降低，且以人参皂苷Rg1与乌头碱2 ∶ 1配伍时作用最强；乌头碱组和各配伍组细胞Na+-K+-ATP酶活力均显著降低，差异均有统计学意义（P＜0.05）；各给药组细胞培养液中ACP、BNP活力均有降低的趋势，细胞内总糖原含量和培养液中TNF-α含量无显著变化（P＞0.05）。结论：人参皂苷Rg1配伍乌头碱可改善大鼠心衰模型心肌细胞ATP酶活力和膜通透性，调节细胞分泌BNP，对心衰模型心肌细胞有一定保护作用，且以人参皂苷Rg1与乌头碱2 ∶ 1配伍时作用最强。 |
| ABSTRACT： | OBJECTIVE： To investigate the protective effect of the compatibilities of ginsenosides Rg1 and aconitine on myocardial cell of in vitro cultured heart failure model. METHODS： The myocardial cells of neonate rat were grouped into normal control group， model group， positive control group （Deslanoside injection， 1×10－7 mol/L）， ginsenosides Rg1 group （1×10－8 mol/L）， aconitine group （1×10－9 mol/L） or their compatibilities groups （1 ∶ 1，2 ∶ 1，1 ∶ 2， V/V）. Except for normal control group， other groups were given 0.8％ pentobarbital sodium to induce heart failure model of myocardial cells. After modeling， each group was given relevant medicine for 1 h， and then the activities of T-ATPase， Ca2+-Mg2+-ATPase， Na+-K+-ATPase in cells were all detected. The activities of acyl carrier protein （ACP） and lactate dehydrogenase （LDH）， and the contents of brain natriuretic party（BNP）， TNF-α and total glycogen were measured in cell culture fluid. RESULTS： Compared with normal control group， T-ATPase and Ca2+-Mg2+-ATPase activities were decreased significantly in model group； meanwhile， Na+-K+-ATPase activity was increased significantly， and ACP， LDH activities and BNP content in cell culture fluid were increased significantly （P＜0.05）. Compared with model group， the activities of T-ATPase in treatment groups were increased significantly， while the activity of LDH in cell culture fluid was decreased significantly； when the volume ratio of ginsenoside Rg1 to aconitine was 2 ∶ 1， protective effect was the strongest； the activities of Na+-K+-ATPase in aconitine group and compatibility groups were all decreased significantly， with statistical significance （P＜0.05）. The activities of ACP and BNP in cell culture fluid were all decreased in treatment groups， but the content of total glycogen in cells and the TNF-α content of in cell culture fluid had no change （P＞0.05）. CONCLUSIONS： Compatibility of ginsenosides Rg1 and aconitine can improve ATPase activities and membranous permeability， regulate BNP secretion and protect myocardial cell of heart failure model， especially the compatibility of ginsenosides Rg1 to aconitine of 2 ∶ 1 ratio. |
| 期刊： | 2017年第28卷第4期 |
| 作者： | 董艳红，谢晓芳，李雪梅，朱雅宁，彭成 |
| AUTHORS： | DONG Yanhong，XIE Xiaofang，LI Xuemei，ZHU Yaning，PENG Cheng |
| 关键字： | 人参皂苷Rg1；乌头碱；配伍；心衰模型；心肌细胞；细胞膜通透性；离子转运相关酶 |
| KEYWORDS： | Ginsenosides Rg1； Aconitine； Compatibility； Heart failure model； Myocardial cell； Membranous permeability； Ion-related ATPase |
| 阅读数： | 397 次 |
| 本月下载数： | 2 次 |
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