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杜仲壮骨丸对维甲酸致小鼠骨质疏松的改善作用
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| 篇名： | 杜仲壮骨丸对维甲酸致小鼠骨质疏松的改善作用 |
| TITLE： | |
| 摘要： | 目的：考察杜仲壮骨丸（DZ）对小鼠骨质疏松的改善作用。方法：将72只KM小鼠随机分为空白对照组（生理盐水）、模型组（生理盐水）、阳性对照组（仙灵骨葆胶囊，0.50 g/kg）和DZ高、中、低剂量组（1.90、0.95、0.48 g/kg，记为DZ-H、DZ-M、DZ-L组），每组12只。除空白对照组外，其余各组小鼠均ig维甲酸复制骨质疏松模型，并同时ig相应药物，每天1次，连续14 d。末次给药后，测定小鼠体质量；摘眼球取血测定血清钙、血清磷含量；取肝、脾、胸腺测定脏器指数；取左侧股骨测定骨湿质量、干质量、长度和直径；取右侧股骨测定骨小梁面积百分比、骨小梁宽度及数目。结果：与空白对照组比较，模型组小鼠体质量降低；血清钙、血清磷含量增加；肝指数、脾指数升高，胸腺指数降低；股骨湿、干质量及直径减少；骨小梁面积百分比降低，骨小梁宽度及数目减少，差异均有统计学意义（P＜0.05或P＜0.01）。与模型组比较，各给药组小鼠血清钙含量减少，阳性对照组和DZ-M、DZ-L组小鼠血清磷含量减少；阳性对照组和DZ-M、DZ-L组小鼠胸腺指数升高，阳性对照组小鼠肝脏指数降低；阳性对照组小鼠的股骨湿质量及DZ-M组小鼠股骨干、湿质量和直径均增加；阳性对照组和DZ-H、DZ-M组小鼠股骨骨小梁百分比升高，骨小梁宽度及骨小梁数目均增加，且DZ-L组小鼠骨小梁数目增加，以上差异均有统计学意义（P＜0.05或P＜0.01）。结论：DZ对维甲酸致小鼠骨质疏松具有一定的改善作用。 |
| ABSTRACT： | OBJECTIVE： To investigate improvement effect of Duzhong zhuanggu （DZ） pills on retinoic acid-induced osteoporosis in mice. METHODS： 72 KM mice were randomized into blank control group （normal saline）， model group （normal saline）， positive control group （Xianling gubao capsule， 0.50 g/kg）， DZ pills high-dose， medium-dose and low-dose groups （1.90，0.95，0.48 g/kg， DZ-H， DZ-M， DZ-L groups）， with 12 mice in each group. Except for blank control group， other groups were given retinoic acid intragastrically to induce osteoporosis model， and given relevant medicine intragastrically once a day， for 14 days. After last administration， body weight of mice was determined； the blood sample was collected from eyeball to determine the contents of serum calcium and phosphor； liver， spleen and thymus gland were collected to determine organ index； left femur was collected to determine bone wet weight， bone dry weight， length and diameter； right femur was collected to determine the area percentage， width and number of bone trabecula. RESULTS： Compared with blank control group， the body weight of mice in model group were decreased； the contents of serum calcium and phosphorus were increased； liver indexes and spleen indexes were increased， while thymus indexes were decreased； bone wet weight， bone dry weight， length and diameter were decreased； area percentage， width and number of trabecular were decreased， with statistical significance （P＜0.05 or P＜0.01）. Compared with model group， the contents of serum calcium were decreased in treatment groups， and the contents of serum phosphorus were decreased in positive control group， DZ-M and DZ-L groups. Thymus indexes were increased in positive control group， DZ-M and DZ-L groups， while liver indexes were decreased in positive control group； femur wet weight of positive control group as well as femur dry and wet weight， diameters of DZ-M were all increased. The percentage， width and number of bone trabecula were increased in positive control group， DZ-H and DZ-M group， and the number of bone trabecula was increased in DZ-L group， with statistical significance （P＜0.05 or P＜0.01）. CONCLUSIONS： DZ pills have improvement effect on osteoporosis induced by retinoic acid in mice. |
| 期刊： | 2017年第28卷第1期 |
| 作者： | 刘明，宣振华，张永萍，刘毅，刘莉，王海洋 |
| AUTHORS： | LIU Ming，XUAN Zhenhua，ZHANG Yongping，LIU Yi，LIU Li，WANG Haiyang |
| 关键字： | 杜仲壮骨丸；维甲酸；骨质疏松模型；小鼠；股骨计量学指标 |
| KEYWORDS： | Duzhong zhuanggu pills； Retinoic acid； Osteoporosis model； Mice； Metrology index of femur |
| 阅读数： | 442 次 |
| 本月下载数： | 27 次 |
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